{"id":"4ef7303b-dd00-58b1-9fe4-23d5311323da","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-endothelial-nox1","predicate":"decreases_expression","statement":"Ergothioneine decreased NOX1 expression in the human endothelial-cell study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"eccabf57-dadc-51c1-8aa2-eb2c989b137d","mechanism_event_label":"The response included a lower oxidant-generating enzyme signal.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"2978fe41-7edd-5786-a167-e7e7eb68c7bf","slug":"nox1","display_name":"Human NADPH oxidase 1 / NOX1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"eccabf57-dadc-51c1-8aa2-eb2c989b137d","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-endothelial-nox1-event","event_type":"observed_relationship","label":"The response included a lower oxidant-generating enzyme signal.","description":"Ergothioneine decreased NOX1 expression in the human endothelial-cell study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2978fe41-7edd-5786-a167-e7e7eb68c7bf","slug":"nox1","display_name":"Human NADPH oxidase 1 / NOX1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human brain microvascular endothelial cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression change, not demonstrated direct NOX1 inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The response included a lower oxidant-generating enzyme signal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Uptake and protective effects of ergothioneine in human endothelial cells. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25022513/ · DOI 10.1124/jpet.114.214049","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4344844e-3bc9-5f86-ba06-627a1e2ea7ad","evidence_kind":"source_excerpt","locator":"Lines 288-294","start_line":288,"end_line":294,"excerpt":"## ergothioneine-endothelial-nox1\nThe response included a lower oxidant-generating enzyme signal.\nErgothioneine decreased NOX1 expression in the human endothelial-cell study.\nModel: Human brain microvascular endothelial cells.\nLimitations: Expression change, not demonstrated direct NOX1 inhibition.\nEvidence access: Primary abstract\nUptake and protective effects of ergothioneine in human endothelial cells. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25022513/ · DOI 10.1124/jpet.114.214049","model_system":"Human brain microvascular endothelial cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}