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(2012). https://pubmed.ncbi.nlm.nih.gov/22523068/ DOI: 10.1172/jci45890","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Osteoblast extracellular matrix","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0879c92c-489f-5acd-a1a1-1135c0bafdc7","evidence_kind":"source_excerpt","locator":"Lines 825-839","start_line":825,"end_line":839,"excerpt":"### vdm-tnap-rescues-calcitriol-culture-mineralization\nAdded TNAP restored mineralization in calcitriol-treated differentiated mouse osteoblast cultures while lowering extracellular pyrophosphate.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Removing the pyrophosphate brake restored mineral deposition in the treated cultures.\norganism: Mus musculus\ntissue_or_cell_type: Osteoblast extracellular matrix\nexperimental_model: Differentiated primary mouse osteoblasts; Alizarin red mineralization and conditioned-medium pyrophosphate assays\nlimitations: Enzyme-addition culture rescue supports pyrophosphate involvement; it does not quantify human bone outcomes or establish the contribution of each calcitriol-regulated gene.\nexposure: 10^-8 M calcitriol and 1 U/mL added TNAP for 4 days; companion cultures tested 5 x 10^-6 M pyrophosphate.\ncross_nutrient: Vitamin D–calcium–phosphate regulation.\nevidence_locator: Figure 6 and adjacent Results: mineralization restored by TNAP\nnutrient: Vitamin D2 and D3\nevidence_scope: D3 active metabolite or VDR machinery experiment; not a direct D2-versus-D3 comparison.\n[vdm-lieben2012] Normocalcemia is maintained in mice under conditions of calcium malabsorption by vitamin D-induced inhibition of bone mineralization. (2012). https://pubmed.ncbi.nlm.nih.gov/22523068/ DOI: 10.1172/jci45890","model_system":"Differentiated primary mouse osteoblasts; Alizarin red mineralization and conditioned-medium pyrophosphate assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [vdm-lieben2012] Normocalcemia is maintained in mice under conditions of calcium malabsorption by vitamin D-induced inhibition of bone mineralization. (2012). https://pubmed.ncbi.nlm.nih.gov/22523068/ DOI: 10.1172/jci45890","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c95c763c-5b31-5b3f-a2f8-2dfe5ef0d3ca","stable_key":"import-1deb434a-3547-51a0-a038-87b1ce79ec38","title":"Vitamin D2 and D3: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"51004bf0d78db6146b8f198b0688a3cc7d4ea9822415a9c034f4c8790fb4e38b","revision_id":"d52ff207-a311-5f24-96f4-462838792db5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}