{"id":"4e5c8051-3265-59d5-8017-64c4c3a43984","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-ado-mouse-enzyme","predicate":"converts_to","statement":"Recombinant mouse Ado oxidized cysteamine to hypotaurine but did not oxidize cysteine in the tested assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e72a2118-7ff3-5bc4-b903-31f32f6e998c","mechanism_event_label":"A separate enzyme provides a route from cysteamine.","subject":{"id":"c232bf42-e787-5555-bae0-7c2302e13297","slug":"mouse-ado","display_name":"Mouse cysteamine dioxygenase / Ado","entity_type_key":"protein"},"object":{"id":"eeff5174-9aa8-5f34-b107-e826edde648c","slug":"hypotaurine","display_name":"Hypotaurine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"e72a2118-7ff3-5bc4-b903-31f32f6e998c","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-ado-mouse-enzyme-event","event_type":"observed_relationship","label":"A separate enzyme provides a route from cysteamine.","description":"Recombinant mouse Ado oxidized cysteamine to hypotaurine but did not oxidize cysteine in the tested assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c232bf42-e787-5555-bae0-7c2302e13297","slug":"mouse-ado","display_name":"Mouse cysteamine dioxygenase / Ado","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"eeff5174-9aa8-5f34-b107-e826edde648c","slug":"hypotaurine","display_name":"Hypotaurine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1149e9db-2832-5f4d-aa5b-5aae11bc7788","slug":"cysteamine","display_name":"Cysteamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant murine protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not assign the mouse enzyme kinetics directly to human ADO.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A separate enzyme provides a route from cysteamine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17581819/ · DOI 10.1074/jbc.M703089200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f4872bbf-7da9-5d44-b59d-269c0d6fb287","evidence_kind":"source_excerpt","locator":"Lines 57-63","start_line":57,"end_line":63,"excerpt":"## taurine-ado-mouse-enzyme\nA separate enzyme provides a route from cysteamine.\nRecombinant mouse Ado oxidized cysteamine to hypotaurine but did not oxidize cysteine in the tested assay.\nModel: Purified recombinant murine protein.\nLimitations: Do not assign the mouse enzyme kinetics directly to human ADO.\nEvidence access: Primary abstract\nDiscovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17581819/ · DOI 10.1074/jbc.M703089200","model_system":"Purified recombinant murine protein.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c6cb77619a374d9b1b04830032fdd3b33d269d160df5baba3778fad5adf57db5","revision_id":"cc45c491-cc6a-5426-ab72-d7c6d26c3614","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}