{"id":"4dec6362-4f9b-5fe7-bc62-7bbe7346e28e","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-intestinal-ace2","predicate":"supports","statement":"Ace2 deletion removed intestinal B0AT1 expression and abolished sodium-dependent isoleucine uptake in mouse ileum segments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"18656f0b-ffc4-5f74-9972-c32f8437a303","mechanism_event_label":"A working transporter also needs its accessory protein in this tissue.","subject":{"id":"51b5a82d-32e8-5223-b384-026dd7cead16","slug":"mouse-ace2","display_name":"Mouse angiotensin-converting enzyme 2 / Ace2","entity_type_key":"protein"},"object":{"id":"9630d0f6-8e87-5ee9-9247-c2de5e1a4a29","slug":"mouse-ileal-isoleucine-uptake","display_name":"Isoleucine uptake in mouse ileum","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"18656f0b-ffc4-5f74-9972-c32f8437a303","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-intestinal-ace2-event","event_type":"observed_relationship","label":"A working transporter also needs its accessory protein in this tissue.","description":"Ace2 deletion removed intestinal B0AT1 expression and abolished sodium-dependent isoleucine uptake in mouse ileum segments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"51b5a82d-32e8-5223-b384-026dd7cead16","slug":"mouse-ace2","display_name":"Mouse angiotensin-converting enzyme 2 / Ace2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9630d0f6-8e87-5ee9-9247-c2de5e1a4a29","slug":"mouse-ileal-isoleucine-uptake","display_name":"Isoleucine uptake in mouse ileum","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b987295b-26b7-5c9a-a033-c5e6ce272186","slug":"mouse-slc6a19","display_name":"Mouse B0AT1 / Slc6a19","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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unverified.","entity":null}],"evidence":[{"id":"c31aca1c-1040-57ae-9ac4-6e3547c3025d","evidence_kind":"source_excerpt","locator":"Lines 26-32","start_line":26,"end_line":32,"excerpt":"## isoleucine-intestinal-ace2\nA working transporter also needs its accessory protein in this tissue.\nAce2 deletion removed intestinal B0AT1 expression and abolished sodium-dependent isoleucine uptake in mouse ileum segments.\nModel: Ace2-null mouse intestine; expression, interaction and uptake assays.\nLimitations: Kidney accessory-protein dependence differs; not evidence that every change in ACE2 produces dietary isoleucine deficiency.\nEvidence access: Primary full text\nTissue-specific amino acid transporter partners ACE2 and collectrin differentially interact with hartnup mutations. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19185582/ · DOI 10.1053/j.gastro.2008.10.055","model_system":"Ace2-null mouse intestine; expression, interaction and uptake assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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