{"id":"4da7e75b-647f-54be-9957-561e0137a68e","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-lat1-partner","predicate":"with_partner_transports","statement":"Human LAT1 coexpressed with human SLC3A2/4F2hc in Xenopus oocytes transported large neutral amino acids; extracellular substrates exchanged intracellular leucine and glutamine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d96fe9fc-4edc-52a3-9911-65215a46feb9","mechanism_event_label":"Transport depends on a partner protein and amino acids on both sides of the membrane.","subject":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"object":{"id":"6281fb5f-b44c-5be1-9fb2-88a809926dc5","slug":"human-lat1-neutral-aa-exchange","display_name":"Human LAT1 neutral amino acid exchange","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d96fe9fc-4edc-52a3-9911-65215a46feb9","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-lat1-partner-event","event_type":"observed_relationship","label":"Transport depends on a partner protein and amino acids on both sides of the membrane.","description":"Human LAT1 coexpressed with human SLC3A2/4F2hc in Xenopus oocytes transported large neutral amino acids; extracellular substrates exchanged intracellular leucine and glutamine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6281fb5f-b44c-5be1-9fb2-88a809926dc5","slug":"human-lat1-neutral-aa-exchange","display_name":"Human LAT1 neutral amino acid exchange","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1ca4b4d5-616a-54d3-8244-0dc151b1bcfe","slug":"slc3a2","display_name":"4F2hc / SLC3A2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human transporter proteins in frog oocytes; human T24-cell disulfide-linked complex also detected.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is an exchange assay, not a direct human brain-uptake trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Transport depends on a partner protein and amino acids on both sides of the membrane.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Human L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11557028/ · DOI 10.1016/s0005-2736(01)00384-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"acca8669-e4b2-5de1-9ecc-24cb72e7dd26","evidence_kind":"source_excerpt","locator":"Lines 42-48","start_line":42,"end_line":48,"excerpt":"## tryptophan-lat1-partner\nTransport depends on a partner protein and amino acids on both sides of the membrane.\nHuman LAT1 coexpressed with human SLC3A2/4F2hc in Xenopus oocytes transported large neutral amino acids; extracellular substrates exchanged intracellular leucine and glutamine.\nModel: Human transporter proteins in frog oocytes; human T24-cell disulfide-linked complex also detected.\nLimitations: This is an exchange assay, not a direct human brain-uptake trial.\nEvidence access: Primary abstract\nHuman L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11557028/ · DOI 10.1016/s0005-2736(01)00384-4","model_system":"Human transporter proteins in frog oocytes; human T24-cell disulfide-linked complex also detected.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}