{"id":"4d2afd65-2d2d-53cd-9513-7084806d6187","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-glucl-cloning","predicate":"gates","statement":"An avermectin-sensitive glutamate-gated chloride channel was cloned from Caenorhabditis elegans and shown to be a new member of the ligand-gated ion channel superfamily.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"13fe6081-5fbb-5564-b94a-43e21a8a2678","mechanism_event_label":"An avermectin-sensitive glutamate-gated chloride channel was cloned from Caenorhabditis elegans and shown to be a new member of the ligand-gated ion channel superfamily.","subject":{"id":"1bafa49b-3d92-5083-bff2-ab29cab57cb4","slug":"avermectin","display_name":"Avermectin","entity_type_key":"chemical_species"},"object":{"id":"fc88a4d0-ca7b-5010-9741-8ea62d0894f3","slug":"celegans-glucl","display_name":"Caenorhabditis elegans glutamate-gated chloride channel alpha / GluCl","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"13fe6081-5fbb-5564-b94a-43e21a8a2678","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-glucl-cloning-event","event_type":"observed_relationship","label":"An avermectin-sensitive glutamate-gated chloride channel was cloned from Caenorhabditis elegans and shown to be a new member of the ligand-gated ion channel superfamily.","description":"An avermectin-sensitive glutamate-gated chloride channel was cloned from Caenorhabditis elegans and shown to be a new member of the ligand-gated ion channel superfamily.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1bafa49b-3d92-5083-bff2-ab29cab57cb4","slug":"avermectin","display_name":"Avermectin","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fc88a4d0-ca7b-5010-9741-8ea62d0894f3","slug":"celegans-glucl","display_name":"Caenorhabditis elegans glutamate-gated chloride channel alpha / GluCl","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Caenorhabditis elegans complementary DNAs expressed in Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Avermectin applied to the expressed channel","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The same report notes that avermectins also interact with vertebrate and invertebrate GABA receptors, so an invertebrate-only target cannot be inferred from this channel alone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Caenorhabditis elegans complementary DNAs expressed in Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An avermectin-sensitive glutamate-gated chloride channel was cloned from Caenorhabditis elegans and shown to be a new member of the ligand-gated ion channel superfamily.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cloning of an avermectin-sensitive glutamate-gated chloride channel from Caenorhabditis elegans. (1994). https://pubmed.ncbi.nlm.nih.gov/7935817/ DOI: 10.1038/371707a0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Expression cloning and electrophysiology of a glutamate-gated chloride channel","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ef10488d-dd39-5cf3-be27-7d0b4c835bb5","evidence_kind":"source_excerpt","locator":"Lines 13-22","start_line":13,"end_line":22,"excerpt":"## ivermectin-glucl-cloning\nAn avermectin-sensitive glutamate-gated chloride channel was cloned from Caenorhabditis elegans and shown to be a new member of the ligand-gated ion channel superfamily.\nModel/species: Caenorhabditis elegans complementary DNAs expressed in Xenopus oocytes\nTissue/system: Expression cloning and electrophysiology of a glutamate-gated chloride channel\nExposure: Avermectin applied to the expressed channel\nRoute: In vitro\nDuration: Acute\nLimits: The same report notes that avermectins also interact with vertebrate and invertebrate GABA receptors, so an invertebrate-only target cannot be inferred from this channel alone.\nPrimary reference: Cloning of an avermectin-sensitive glutamate-gated chloride channel from Caenorhabditis elegans. (1994). https://pubmed.ncbi.nlm.nih.gov/7935817/ DOI: 10.1038/371707a0\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"20818ddd-7b37-5226-a24b-7de007e80eb2","stable_key":"import-e0801c92-7fd3-5cb2-82b7-dd82f010f3ed","title":"Ivermectin: mechanism of action across parasite, host barrier and mammalian targets (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of sixteen primary studies resolved by PubMed title search and cross-checked against live PubMed metadata. Study-specific citations, concentrations, negative findings and limitations retained. 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