{"id":"4cee7a7e-ac65-53ac-a522-f1cc4710a23f","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-copper-dopamine-oxidation","predicate":"inhibits_in_assay","statement":"Theanine suppressed copper-facilitated dopamine oxidation; the authors attributed this to copper chelation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ef1265f4-973b-5d7a-9ef5-4b2e829ebfd3","mechanism_event_label":"Copper changed the chemistry of dopamine oxidation, and theanine interfered with that chemistry in the assay.","subject":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"object":{"id":"67eded54-1836-58a7-9b48-8b2e526f4371","slug":"copper-mediated-dopamine-oxidation","display_name":"Copper-facilitated dopamine oxidation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ef1265f4-973b-5d7a-9ef5-4b2e829ebfd3","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-copper-dopamine-oxidation-event","event_type":"biochemical_relationship","label":"Copper changed the chemistry of dopamine oxidation, and theanine interfered with that chemistry in the assay.","description":"Theanine suppressed copper-facilitated dopamine oxidation; the authors attributed this to copper chelation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9f0afdde-1ec1-5c8a-bb5e-f3b2b75f67f6","slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"},"role":"metal_context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9cca6209-0603-5ab7-96ad-2f3ae4b772d2","slug":"dopamine","display_name":"Dopamine","entity_type_key":"small_molecule"},"role":"oxidation_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"67eded54-1836-58a7-9b48-8b2e526f4371","slug":"copper-mediated-dopamine-oxidation","display_name":"Copper-facilitated dopamine oxidation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/39499422.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"abb7a869056bc8c43d6792b2a4ff9a1b15738facf454daeb3f92df383932b9c4\", \"start_char\": 0, \"end_char\": 1871, \"text_sha256\": \"abb7a869056bc8c43d6792b2a4ff9a1b15738facf454daeb3f92df383932b9c4\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free oxidation and cultured-cell injury assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dopamine with and without copper; theanine concentration-ratio experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High experimental substrate ratios; no human copper-depletion, Parkinson disease treatment or systemic chelation conclusion. Cellular protection does not establish effective brain exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human SH-SY5Y cell line and chemical systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Copper changed the chemistry of dopamine oxidation, and theanine interfered with that chemistry in the assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p39499422] L-Theanine Effectively Protects Against Copper-Facilitated Dopamine Oxidation: Implication for Relieving Dopamine Overflow-Associated Neurotoxicities. (2025). https://pubmed.ncbi.nlm.nih.gov/39499422/ DOI: 10.1007/s12035-024-04601-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Copper-facilitated dopamine oxidation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1d49a4da-30d9-5da3-b3a7-4173fe69c4f8","evidence_kind":"source_excerpt","locator":"Lines 731-742","start_line":731,"end_line":742,"excerpt":"### theanine-copper-dopamine-oxidation\nTheanine suppressed copper-facilitated dopamine oxidation; the authors attributed this to copper chelation.\nCondition category: normal\nnutrient_topic: L-Theanine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Copper changed the chemistry of dopamine oxidation, and theanine interfered with that chemistry in the assay.\norganism: Human SH-SY5Y cell line and chemical systems\ntissue_or_cell_type: Copper-facilitated dopamine oxidation\nexperimental_model: Cell-free oxidation and cultured-cell injury assays\nlimitations: High experimental substrate ratios; no human copper-depletion, Parkinson disease treatment or systemic chelation conclusion. Cellular protection does not establish effective brain exposure.\nexposure: Dopamine with and without copper; theanine concentration-ratio experiments\nevidence_span: {\"source_cache\": \"artifacts/theanine-research/39499422.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"abb7a869056bc8c43d6792b2a4ff9a1b15738facf454daeb3f92df383932b9c4\", \"start_char\": 0, \"end_char\": 1871, \"text_sha256\": \"abb7a869056bc8c43d6792b2a4ff9a1b15738facf454daeb3f92df383932b9c4\"}\n[theanine-p39499422] L-Theanine Effectively Protects Against Copper-Facilitated Dopamine Oxidation: Implication for Relieving Dopamine Overflow-Associated Neurotoxicities. (2025). https://pubmed.ncbi.nlm.nih.gov/39499422/ DOI: 10.1007/s12035-024-04601-x","model_system":"Cell-free oxidation and cultured-cell injury assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [theanine-p39499422] L-Theanine Effectively Protects Against Copper-Facilitated Dopamine Oxidation: Implication for Relieving Dopamine Overflow-Associated Neurotoxicities. (2025). https://pubmed.ncbi.nlm.nih.gov/39499422/ DOI: 10.1007/s12035-024-04601-x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2bf3fc8-fc9a-5d50-937a-559a76f8be7d","stable_key":"import-8f12ba85-1a92-5337-a9c0-9cee9b3be90d","title":"L-Theanine: metabolism, neural signaling, nutrient connections and human outcomes (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"c78f2a1961da546cd8d3ae9131a16613aed0d1032904ca19175b0641647142c1","revision_id":"4ec64995-1e5b-5279-9102-f203cb2e29e5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}