{"id":"4cd7ae21-b97b-55b0-b0ee-9707737e88b7","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-rpe-carbon","predicate":"supplies","statement":"Cultured human fetal RPE consumed proline preferentially among the measured nutrients and used its carbon in the TCA cycle and reductive citrate synthesis, with preferential apical citrate export.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"3599f7db-ee65-5610-a27e-3318b530a159","mechanism_event_label":"The eye support layer can turn proline into fuel intermediates for its neighbors.","subject":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"object":{"id":"bbe05380-5a34-5c8b-8606-d8d3f308a397","slug":"human-rpe-proline-carbon-export","display_name":"Proline-derived carbon metabolism and apical export by human RPE","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3599f7db-ee65-5610-a27e-3318b530a159","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-rpe-carbon-event","event_type":"observed_relationship","label":"The eye support layer can turn proline into fuel intermediates for its neighbors.","description":"Cultured human fetal RPE consumed proline preferentially among the measured nutrients and used its carbon in the TCA cycle and reductive citrate synthesis, with preferential apical citrate export.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bbe05380-5a34-5c8b-8606-d8d3f308a397","slug":"human-rpe-proline-carbon-export","display_name":"Proline-derived carbon metabolism and apical export by human RPE","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Polarized human fetal RPE culture, carbon-13 tracing and metabolite measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Culture preferences do not establish a universal human dietary proline requirement or AMD treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The eye support layer can turn proline into fuel intermediates for its neighbors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Human retinal pigment epithelial cells prefer proline as a nutrient and transport metabolic intermediates to the retinal side. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28615447/ · DOI 10.1074/jbc.M117.788422","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"70efb557-48fa-5f9a-b88e-86c52cd93f95","evidence_kind":"source_excerpt","locator":"Lines 302-308","start_line":302,"end_line":308,"excerpt":"## l-proline-rpe-carbon\nThe eye support layer can turn proline into fuel intermediates for its neighbors.\nCultured human fetal RPE consumed proline preferentially among the measured nutrients and used its carbon in the TCA cycle and reductive citrate synthesis, with preferential apical citrate export.\nModel: Polarized human fetal RPE culture, carbon-13 tracing and metabolite measurements.\nLimitations: Culture preferences do not establish a universal human dietary proline requirement or AMD treatment.\nEvidence access: Primary abstract\nHuman retinal pigment epithelial cells prefer proline as a nutrient and transport metabolic intermediates to the retinal side. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28615447/ · DOI 10.1074/jbc.M117.788422","model_system":"Polarized human fetal RPE culture, carbon-13 tracing and metabolite measurements.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7b7f1981f9c7897fbb6baa19425bdc19dede78ad4d66fa554394b8337c7366fb","revision_id":"4971a925-fa70-59f8-8030-d3029a18a62f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}