{"id":"4cd07c71-1298-5232-8375-7ab9cc01f60e","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-svct2-uptake","predicate":"transports","statement":"Rat SVCT2 expressed in Xenopus oocytes mediated stereospecific, concentrative uptake of reduced L-ascorbate driven by the sodium electrochemical gradient.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d28aa4fe-bcb2-5923-85b3-a81483970f17","mechanism_event_label":"SVCT2 uses a sodium gradient to bring reduced vitamin C into cells.","subject":{"id":"d8382cc1-953f-505d-8887-18b39504b0ec","slug":"rat-slc23a2","display_name":"Rat sodium-dependent vitamin C transporter 2 / Slc23a2","entity_type_key":"protein"},"object":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"d28aa4fe-bcb2-5923-85b3-a81483970f17","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-svct2-uptake-event","event_type":"biochemical_relationship","label":"SVCT2 uses a sodium gradient to bring reduced vitamin C into cells.","description":"Rat SVCT2 expressed in Xenopus oocytes mediated stereospecific, concentrative uptake of reduced L-ascorbate driven by the sodium electrochemical gradient.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"coupled ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d8382cc1-953f-505d-8887-18b39504b0ec","slug":"rat-slc23a2","display_name":"Rat sodium-dependent vitamin C transporter 2 / Slc23a2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat cDNA transporters expressed in Xenopus oocytes; mammalian tissue distribution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Heterologous cDNA expression and uptake assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Primary abstract only; no inferred human kinetic constants.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus protein in Xenopus laevis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SVCT2 uses a sodium gradient to bring reduced vitamin C into cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[tsukaguchi1999] A family of mammalian Na+-dependent L-ascorbic acid transporters. (1999). https://pubmed.ncbi.nlm.nih.gov/10331392/ DOI: 10.1038/19986","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Oocyte plasma membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Stereospecific concentrative uptake driven by the sodium electrochemical gradient.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Stereospecific concentrative uptake driven by the sodium electrochemical gradient.","entity":null}],"evidence":[{"id":"73672790-6f06-5ad8-a6f0-ad89cf1192ba","evidence_kind":"source_excerpt","locator":"Lines 117-128","start_line":117,"end_line":128,"excerpt":"### vc-transport-svct2-uptake\nRat SVCT2 expressed in Xenopus oocytes mediated stereospecific, concentrative uptake of reduced L-ascorbate driven by the sodium electrochemical gradient.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: SVCT2 uses a sodium gradient to bring reduced vitamin C into cells.\norganism: Rattus norvegicus protein in Xenopus laevis\ntissue_or_cell_type: Oocyte plasma membrane\nexperimental_model: Rat cDNA transporters expressed in Xenopus oocytes; mammalian tissue distribution\nlimitations: Primary abstract only; no inferred human kinetic constants.\nexposure: Heterologous cDNA expression and uptake assays\ncross_nutrient: true\n[tsukaguchi1999] A family of mammalian Na+-dependent L-ascorbic acid transporters. (1999). https://pubmed.ncbi.nlm.nih.gov/10331392/ DOI: 10.1038/19986","model_system":"Rat cDNA transporters expressed in Xenopus oocytes; mammalian tissue distribution","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [tsukaguchi1999] A family of mammalian Na+-dependent L-ascorbic acid transporters. (1999). https://pubmed.ncbi.nlm.nih.gov/10331392/ DOI: 10.1038/19986","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fa811221-13bd-5c10-adc1-eaf097c7703c","stable_key":"import-cb568d28-484a-5c2e-9fcc-2d780358e514","title":"Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b7fd83f956abb81855f2ea23199ba14e465cd91f4a2ac560277ec21ddafd7bfc","revision_id":"6cde9bbb-d712-5746-82a6-85b144253efa","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}