{"id":"4cc4a063-3a0e-54d6-98b3-1e65cbb9f3a1","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:gssa-growth-rescue","predicate":"increases_in_recorded_experiment","statement":"GSSA supplementation supported growth of GSH1-deficient yeast on otherwise glutathione-unsupplemented medium.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"92a67ea0-51a9-59f4-be18-f758b3c6021e","mechanism_event_label":"GSSA supplementation supported growth of GSH1-deficient yeast on otherwise glutathione-unsupplemented medium.","subject":{"id":"abc6eee3-1846-5180-9cc9-93379e6a02c9","slug":"s-allylmercaptoglutathione","display_name":"S-Allylmercaptoglutathione / GSSA","entity_type_key":"small_molecule"},"object":{"id":"39f3754f-9a34-508f-aac3-7b981148f80b","slug":"yeast-gsh1-deficient-growth","display_name":"Growth of yeast lacking GSH1","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"92a67ea0-51a9-59f4-be18-f758b3c6021e","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:gssa-growth-rescue-event","event_type":"experimental_observation","label":"GSSA supplementation supported growth of GSH1-deficient yeast on otherwise glutathione-unsupplemented medium.","description":"**GSSA recycling and NADPH.** Purified yeast glutathione reductase reduced GSSA with NADPH consumption. Reported apparent Km values were 0.50 mM for GSSA and 0.07 mM for GSSG. They do not establish an eightfold difference in catalytic efficiency: turnover and assay differences matter, and the GSSA measurement deliberately omitted the DTNB cycling reagent used for GSSG. GSSA also supported growth of glutathione-synthesis-defective yeast. That rescue is consistent with recovery of usable glutathione, but it is not a human GSR experiment or proof that this enzyme was the exclusive route in living cells. [Horn 2018](https://pmc.ncbi.nlm.nih.gov/articles/PMC6070820/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"abc6eee3-1846-5180-9cc9-93379e6a02c9","slug":"s-allylmercaptoglutathione","display_name":"S-Allylmercaptoglutathione / GSSA","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"GSSA supplied to GSH1-deficient yeast","sequence_order":0,"notes":""},{"entity":{"id":"39f3754f-9a34-508f-aac3-7b981148f80b","slug":"yeast-gsh1-deficient-growth","display_name":"Growth of yeast lacking GSH1","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"782a89cb-b15e-5b88-85e3-87ec8036ac1a","slug":"yeast-gsh1","display_name":"Saccharomyces cerevisiae glutamate-cysteine ligase Gsh1","entity_type_key":"protein"},"role":"deleted biosynthetic machinery","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"required cellular thiol","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"supplied","comparator":"GSH1-deficient yeast without exogenous glutathione source","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"s-allylmercaptoglutathione","display_name":"S-Allylmercaptoglutathione / GSSA","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"deleted","comparator":"GSH1-deficient yeast without exogenous glutathione source","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"yeast-gsh1","display_name":"Saccharomyces cerevisiae glutamate-cysteine ligase Gsh1","entity_type_key":"protein"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"GSSA supplied to GSH1-deficient yeast\", \"comparator\": \"GSH1-deficient yeast without exogenous glutathione source\", \"endpoint\": \"GSSA supplementation supported growth of GSH1-deficient yeast on otherwise glutathione-unsupplemented medium.\", \"effect_direction\": \"increase\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"yeast-gsh1\", \"state\": \"deleted\"}, {\"entity_slug\": \"s-allylmercaptoglutathione\", \"state\": \"supplied\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Saccharomyces cerevisiae GSH1 deletion; GSSA compared with unsupplemented growth medium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rescue supports usable GSH recovery but does not prove the exclusive in vivo reductase or human rescue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"GSSA supplementation supported growth of GSH1-deficient yeast on otherwise glutathione-unsupplemented medium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"<i>S</i>-allylmercaptoglutathione Is a Substrate for Glutathione Reductase (E.C. 1.8.1.7) from Yeast (<i>Saccharomyces cerevisiae</i>). | 2018 | DOI 10.3390/antiox7070086 | PMID 29986384 | https://pubmed.ncbi.nlm.nih.gov/29986384/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC6070820/ | https://doi.org/10.3390/antiox7070086","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 47-47; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"e2241a87-41cb-50cc-84cf-3ef775764bc3","evidence_kind":"source_excerpt","locator":"Lines 47-47","start_line":47,"end_line":47,"excerpt":"**GSSA recycling and NADPH.** Purified yeast glutathione reductase reduced GSSA with NADPH consumption. Reported apparent Km values were 0.50 mM for GSSA and 0.07 mM for GSSG. They do not establish an eightfold difference in catalytic efficiency: turnover and assay differences matter, and the GSSA measurement deliberately omitted the DTNB cycling reagent used for GSSG. GSSA also supported growth of glutathione-synthesis-defective yeast. That rescue is consistent with recovery of usable glutathione, but it is not a human GSR experiment or proof that this enzyme was the exclusive route in living cells. [Horn 2018](https://pmc.ncbi.nlm.nih.gov/articles/PMC6070820/)","model_system":"Saccharomyces cerevisiae GSH1 deletion; GSSA compared with unsupplemented growth medium.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}