{"id":"4cb0f965-54c1-507f-9469-16adda2aa017","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-apip-zinc-site","predicate":"requires_in_mutation_test","statement":"Mutation of the predicted zinc-binding site abolished APIP salvage function, whereas three tested potential phosphorylation-site mutations did not.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1639133f-1550-5ad1-886a-d49a4949b1e4","mechanism_event_label":"A metal-binding site can be a separate gate in nutrient recycling.","subject":{"id":"d4e3b8c8-690d-58ba-8a00-99221988daf8","slug":"apip","display_name":"Human methylthioribulose-1-phosphate dehydratase / APIP","entity_type_key":"protein"},"object":{"id":"20fb5c55-54b8-5c48-b1f1-88565f1b4634","slug":"human-apip-zinc-binding-site","display_name":"Putative zinc-binding site of human APIP","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1639133f-1550-5ad1-886a-d49a4949b1e4","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-apip-zinc-site-event","event_type":"observed_relationship","label":"A metal-binding site can be a separate gate in nutrient recycling.","description":"Mutation of the predicted zinc-binding site abolished APIP salvage function, whereas three tested potential phosphorylation-site mutations did not.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d4e3b8c8-690d-58ba-8a00-99221988daf8","slug":"apip","display_name":"Human methylthioribulose-1-phosphate dehydratase / APIP","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"20fb5c55-54b8-5c48-b1f1-88565f1b4634","slug":"human-apip-zinc-binding-site","display_name":"Putative zinc-binding site of human APIP","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"525860da-a986-5d19-97f0-a257a454b649","slug":"5-methylthioadenosine","display_name":"5′-Methylthioadenosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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