{"id":"4ca81500-9661-58f1-ac1e-195eb9daaeed","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-ebf2-cars2-expression","predicate":"induces","statement":"The study reported direct EBF2 induction of Cars2 during brown-adipocyte differentiation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a36ed56f-a3a1-55b8-a859-30eb2b1e6b83","mechanism_event_label":"A transcription factor increased the machinery for a sulfur-signaling pathway.","subject":{"id":"104876de-8ff6-5cf1-940f-1ef80bfbe85e","slug":"mouse-ebf2","display_name":"Mouse early B-cell factor 2 / Ebf2","entity_type_key":"protein"},"object":{"id":"9fe61578-bc3d-54ef-947a-4537a63d47ba","slug":"mouse-cars2","display_name":"Mouse mitochondrial cysteinyl-tRNA synthetase / Cars2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a36ed56f-a3a1-55b8-a859-30eb2b1e6b83","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-ebf2-cars2-expression-event","event_type":"observed_relationship","label":"A transcription factor increased the machinery for a sulfur-signaling pathway.","description":"The study reported direct EBF2 induction of Cars2 during brown-adipocyte differentiation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"104876de-8ff6-5cf1-940f-1ef80bfbe85e","slug":"mouse-ebf2","display_name":"Mouse early B-cell factor 2 / Ebf2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9fe61578-bc3d-54ef-947a-4537a63d47ba","slug":"mouse-cars2","display_name":"Mouse mitochondrial cysteinyl-tRNA synthetase / Cars2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse brown-fat study and cellular differentiation experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract-level extraction; detailed promoter occupancy and construct conditions require full-method review.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Cysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A transcription factor increased the machinery for a sulfur-signaling pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cars2-Mediated Cysteine Catabolism Drives Brown Fat Development and Thermogenesis Through Persulfidating EBF2. · 2026 · https://pubmed.ncbi.nlm.nih.gov/41849685/ · DOI 10.1002/advs.202522690","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c2c9fd10-7a1a-56ee-9838-ce7ecc48f2eb","evidence_kind":"source_excerpt","locator":"Lines 388-394","start_line":388,"end_line":394,"excerpt":"## l-cysteine-ebf2-cars2-expression\nA transcription factor increased the machinery for a sulfur-signaling pathway.\nThe study reported direct EBF2 induction of Cars2 during brown-adipocyte differentiation.\nModel: Mouse brown-fat study and cellular differentiation experiments.\nLimitations: Abstract-level extraction; detailed promoter occupancy and construct conditions require full-method review.\nEvidence access: Primary abstract\nCars2-Mediated Cysteine Catabolism Drives Brown Fat Development and Thermogenesis Through Persulfidating EBF2. · 2026 · https://pubmed.ncbi.nlm.nih.gov/41849685/ · DOI 10.1002/advs.202522690","model_system":"Mouse brown-fat study and cellular differentiation experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"654560f6-8d7f-596b-8722-48d94053cfe3","stable_key":"import-a8baf7e9-80e4-5d8c-adec-9a63e84d2f21","title":"L-Cysteine: sulfur allocation, redox supply and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f722669e54eab08ffe7289f9d79ddfc443014ed8c4bc5ba10ce2635470d22498","revision_id":"8ae25609-a031-5782-9c62-6cad8767ea46","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}