{"id":"4c78c5d4-3819-52af-87ce-b4a7c6ec60a4","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-gsh-interaction","predicate":"interacts_in_cell_free_system_with","statement":"UV spectroscopy and FTICR mass spectrometry detected physical interaction between cucurbitacin B and glutathione in a cell-free system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8b2afc5e-e6da-559f-85c1-f47c0e59fb23","mechanism_event_label":"Glutathione can interact with the compound before a downstream signal is measured.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"8b2afc5e-e6da-559f-85c1-f47c0e59fb23","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-gsh-interaction-event","event_type":"observed_relationship","label":"Glutathione can interact with the compound before a downstream signal is measured.","description":"UV spectroscopy and FTICR mass spectrometry detected physical interaction between cucurbitacin B and glutathione in a cell-free system.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free chemistry; exposure concentration and duration unavailable in the accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Physical interaction is not a quantified in-vivo detoxification rate or human glutathione-depletion threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Glutathione can interact with the compound before a downstream signal is measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin B potently suppresses non-small-cell lung cancer growth: identification of intracellular thiols as critical targets. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23340170/ · DOI 10.1016/j.canlet.2013.01.008","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"42d4b98b-3a29-5f8c-a9a9-d5b201ac6dca","evidence_kind":"source_excerpt","locator":"Lines 108-114","start_line":108,"end_line":114,"excerpt":"## cucurbitacin-b-gsh-interaction\nGlutathione can interact with the compound before a downstream signal is measured.\nUV spectroscopy and FTICR mass spectrometry detected physical interaction between cucurbitacin B and glutathione in a cell-free system.\nModel: Cell-free chemistry; exposure concentration and duration unavailable in the accessed abstract.\nLimitations: Physical interaction is not a quantified in-vivo detoxification rate or human glutathione-depletion threshold.\nEvidence access: Primary abstract\nCucurbitacin B potently suppresses non-small-cell lung cancer growth: identification of intracellular thiols as critical targets. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23340170/ · DOI 10.1016/j.canlet.2013.01.008","model_system":"Cell-free chemistry; exposure concentration and duration unavailable in the accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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