{"id":"4c6ec059-5a30-5fea-ac8e-17b6a897d6c9","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-cyp24a1-active-d2","predicate":"produces","statement":"Human CYP24A1 initiated active 1alpha,25-dihydroxyvitamin D2 oxidation through C24R hydroxylation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"21328ac4-f166-509e-abde-7eb83857ed14","mechanism_event_label":"CYP24A1 also starts breaking down the active D2 hormone.","subject":{"id":"c0a944e7-deb8-5f9e-8428-91496098e38f","slug":"cyp24a1","display_name":"Vitamin D 24-hydroxylase / CYP24A1","entity_type_key":"protein"},"object":{"id":"d1bd9598-1673-50ee-b684-be4e255c9605","slug":"1-24r-25-trihydroxyvitamin-d2","display_name":"1-alpha,24R,25-Trihydroxyvitamin D2","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"21328ac4-f166-509e-abde-7eb83857ed14","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-cyp24a1-active-d2-event","event_type":"biochemical_relationship","label":"CYP24A1 also starts breaking down the active D2 hormone.","description":"Human CYP24A1 initiated active 1alpha,25-dihydroxyvitamin D2 oxidation through C24R hydroxylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c0a944e7-deb8-5f9e-8428-91496098e38f","slug":"cyp24a1","display_name":"Vitamin D 24-hydroxylase / CYP24A1","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"77a8ef8f-1309-58e1-a915-8ba79e0d0038","slug":"1-25-dihydroxyvitamin-d2","display_name":"1-alpha,25-Dihydroxyvitamin D2","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d1bd9598-1673-50ee-b684-be4e255c9605","slug":"1-24r-25-trihydroxyvitamin-d2","display_name":"1-alpha,24R,25-Trihydroxyvitamin D2","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract: identified C24R products and comparative initial-rate kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Partially purified human CYP24A1 product analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"1,25(OH)2D2 substrate; concentration/time not specified in abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Product profile is human enzyme-specific; active D2 is not calcitriol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin D2 and D3","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CYP24A1 also starts breaking down the active D2 hormone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[li2023] Inactivation of vitamin D2 metabolites by human CYP24A1. (2023). https://pubmed.ncbi.nlm.nih.gov/37495192/ DOI: 10.1016/j.jsbmb.2023.106368","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"phospholipid-vesicle membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fac507c0-f5d9-5af1-b912-599812a0c00a","evidence_kind":"source_excerpt","locator":"Lines 527-540","start_line":527,"end_line":540,"excerpt":"### vd-act-cyp24a1-active-d2\nHuman CYP24A1 initiated active 1alpha,25-dihydroxyvitamin D2 oxidation through C24R hydroxylation.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: CYP24A1 also starts breaking down the active D2 hormone.\norganism: Homo sapiens protein\ntissue_or_cell_type: phospholipid-vesicle membrane\nexperimental_model: Partially purified human CYP24A1 product analysis\nlimitations: Product profile is human enzyme-specific; active D2 is not calcitriol.\nexposure: 1,25(OH)2D2 substrate; concentration/time not specified in abstract.\ncross_nutrient: false\nevidence_location: Primary abstract: identified C24R products and comparative initial-rate kinetics.\nnutrient: Vitamin D2 and D3\n[li2023] Inactivation of vitamin D2 metabolites by human CYP24A1. 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