{"id":"4c52dafa-bac4-5f87-9bf1-7cd1500b771b","stable_key":"e054f0f5-417f-5850-a874-91ff2c9de631:sfn-gap43-null","predicate":"has_contextual_experimental_effect","statement":"The sulforaphane study reports no significant change in GAP-43 palmitoylation in the presence of sulforaphane.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"09d2977b-6abf-53d3-b066-65333e2b431b","mechanism_event_label":"sfn gap43 null","subject":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"object":{"id":"3dfcdb61-aca7-53bc-a28f-9e05b747a0c9","slug":"gap43-palmitoylation-readout","display_name":"GAP-43 palmitoylation in the sulforaphane null-result report","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"09d2977b-6abf-53d3-b066-65333e2b431b","stable_key":"e054f0f5-417f-5850-a874-91ff2c9de631:sfn-gap43-null","event_type":"experimental_result","label":"sfn gap43 null","description":"The sulforaphane study reports no significant change in GAP-43 palmitoylation in the presence of sulforaphane.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3dfcdb61-aca7-53bc-a28f-9e05b747a0c9","slug":"gap43-palmitoylation-readout","display_name":"GAP-43 palmitoylation in the sulforaphane null-result report","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_cache","value_text":"artifacts/discovery-research/round6-sources/sulforaphane-apt2.pdf; SHA256 31e2993f88fe05eec199b9013173b29379b3346b9696ac766771173ddd0f181a","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell model and construct species not specified for this discussion-only result","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dose, timing and assay details for this null result are not separately reported.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"These are separate experimental observations, not a demonstrated sulforaphane-GPX4-ZDHHC6 pathway. Sulforaphane-driven APT2 relocalization is not equivalent to genetic depletion or general catalytic inhibition. No dietary, clinical or selenium-repletion effect is inferred. Protein stability, palmitoylation and substrate output remain different measurements. No plotted experiment or effect-size interval is provided. Preserve this author-reported null; it is not proof of equivalence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human experimental cells; construct species unresolved where stated for discussion-only nulls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Discussion, page 6","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1016/j.bbrc.2024.150244","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_access","value_text":"Full six-page primary PDF, methods and discussion read; Figure 4 visually inspected. 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No raw-data reanalysis.\nevidence_cache: artifacts/discovery-research/round6-sources/sulforaphane-apt2.pdf; SHA256 31e2993f88fe05eec199b9013173b29379b3346b9696ac766771173ddd0f181a\nexperimental_model: Cell model and construct species not specified for this discussion-only result\norganism: Human experimental cells; construct species unresolved where stated for discussion-only nulls\nexposure: Dose, timing and assay details for this null result are not separately reported.\nlimitations: These are separate experimental observations, not a demonstrated sulforaphane-GPX4-ZDHHC6 pathway. Sulforaphane-driven APT2 relocalization is not equivalent to genetic depletion or general catalytic inhibition. No dietary, clinical or selenium-repletion effect is inferred. Protein stability, palmitoylation and substrate output remain different measurements. No plotted experiment or effect-size interval is provided. Preserve this author-reported null; it is not proof of equivalence.","model_system":"Cell model and construct species not specified for this discussion-only result","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase and primary locator; not a verbatim publisher quote. Null-result limitations retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d7785cbf-e87c-5662-a76c-7ef11586cca5","stable_key":"import-e054f0f5-417f-5850-a874-91ff2c9de631","title":"APT2: sulforaphane engagement, GPX4 stability and ZDHHC6 turnover","document_type":"imported_text","citation_label":"Targeted primary-literature curation from recursive ZDHHC6 exploration, 2026-09-20.","file_path":"","sha256":"011acbeafd419a6826789806b59b5d7ff312b222762824580036325083586956","revision_id":"663a0bd1-3e46-5fee-ab0f-dcf992b2a809","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}