{"id":"4c0c7d31-f2d6-5476-b5b8-ed17f364a769","stable_key":"3ad23a58-d6a8-5554-81d6-9c83abd33087:znt8-chelation-apoptosis","predicate":"reported_comparison","statement":"After 5 micromolar TPEN for 48 hours, apoptosis was lower in SLC30A8-null and R138X cells than treated wild-type cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"10c0691f-59c1-5910-af99-1422ab5fbd4c","mechanism_event_label":"After 5 micromolar TPEN for 48 hours, apoptosis was lower in SLC30A8-null and R138X cells than treated wild-type cells.","subject":{"id":"5ec5ce4e-ee24-5bff-a525-5e57fc4db7c3","slug":"human-slc30a8-null-beta-cells","display_name":"Human stem-cell-derived SLC30A8-null beta cells","entity_type_key":"cell_type"},"object":{"id":"d9598575-8aaa-5d30-9c9f-84a28fdf69a5","slug":"human-beta-cell-apoptosis","display_name":"Human beta-cell apoptosis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"10c0691f-59c1-5910-af99-1422ab5fbd4c","stable_key":"3ad23a58-d6a8-5554-81d6-9c83abd33087:znt8-chelation-apoptosis","event_type":"reported_comparison","label":"After 5 micromolar TPEN for 48 hours, apoptosis was lower in SLC30A8-null and R138X cells than treated wild-type cells.","description":"","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5ec5ce4e-ee24-5bff-a525-5e57fc4db7c3","slug":"human-slc30a8-null-beta-cells","display_name":"Human stem-cell-derived SLC30A8-null beta cells","entity_type_key":"cell_type"},"role":"experimental_actor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d9598575-8aaa-5d30-9c9f-84a28fdf69a5","slug":"human-beta-cell-apoptosis","display_name":"Human beta-cell apoptosis","entity_type_key":"cellular_process"},"role":"measured_endpoint","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a4da847b-7430-527e-bc73-8d17fd5255ba","slug":"slc30a8","display_name":"Human zinc transporter ZnT8 / SLC30A8","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e0dc1af6-0068-5ae2-8ab0-a5199252ce7d","slug":"human-slc30a8-r138x-beta-cells","display_name":"Human stem-cell-derived SLC30A8 R138X beta cells","entity_type_key":"cell_type"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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R138X tested separately\"}, {\"entity_slug\": \"zinc\", \"state\": \"Limited availability during TPEN exposure\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Engineered human embryonic stem-cell-derived beta cells; results and figure 5 reviewed","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Chelator challenge, not dietary restriction; human in-vivo protection by this mechanism remains untested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"SLC30A8 stem-cell study; 2023; PMCID:PMC10047077; https://pmc.ncbi.nlm.nih.gov/articles/PMC10047077/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"3503f5f9-756b-51d1-af02-e579f8dfe52d","evidence_kind":"source_excerpt","locator":"Lines 15-15","start_line":15,"end_line":15,"excerpt":"After 5 micromolar TPEN for 48 hours, apoptosis was lower in SLC30A8-null and R138X cells than treated wild-type cells. Model: Engineered human embryonic stem-cell-derived beta cells; results and figure 5 reviewed. Limits: Chelator challenge, not dietary restriction; human in-vivo protection by this mechanism remains untested. 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