{"id":"4c056e74-9f58-548b-95da-3de28d039bc3","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-mef-marker-not-killing","predicate":"reduces","statement":"Fisetin reduced senescence markers in stressed Ercc1-deficient mouse fibroblasts without evidence of cell killing in that setting.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"cfa893ba-a380-5f70-a623-72f74e4a6bc9","mechanism_event_label":"A lower senescence signal can occur without killing cells.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"8098f6b5-5ef9-58e5-b155-285c3606f22f","slug":"mouse-ercc1-mef-senescence-markers","display_name":"Senescence markers in Ercc1-deficient mouse embryonic fibroblasts","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cfa893ba-a380-5f70-a623-72f74e4a6bc9","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-mef-marker-not-killing-event","event_type":"observed_relationship","label":"A lower senescence signal can occur without killing cells.","description":"Fisetin reduced senescence markers in stressed Ercc1-deficient mouse fibroblasts without evidence of cell killing in that setting.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8098f6b5-5ef9-58e5-b155-285c3606f22f","slug":"mouse-ercc1-mef-senescence-markers","display_name":"Senescence markers in Ercc1-deficient mouse embryonic fibroblasts","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse fibroblast screening described in the primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not label every marker reduction as senolysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A lower senescence signal can occur without killing cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fisetin is a senotherapeutic that extends health and lifespan. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30279143/ · DOI 10.1016/j.ebiom.2018.09.015","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8fd7124c-f3da-5ac0-8ea5-eb905a9b90a1","evidence_kind":"source_excerpt","locator":"Lines 208-214","start_line":208,"end_line":214,"excerpt":"## fisetin-mef-marker-not-killing\nA lower senescence signal can occur without killing cells.\nFisetin reduced senescence markers in stressed Ercc1-deficient mouse fibroblasts without evidence of cell killing in that setting.\nModel: Mouse fibroblast screening described in the primary study.\nLimitations: Do not label every marker reduction as senolysis.\nEvidence access: Primary full text\nFisetin is a senotherapeutic that extends health and lifespan. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30279143/ · DOI 10.1016/j.ebiom.2018.09.015","model_system":"Mouse fibroblast screening described in the primary study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}