{"id":"4bf253ce-c84a-58bf-bd36-e39f46252d50","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:a-vision-rdh8-reduction","predicate":"produces","statement":"Single-cell knockout comparisons showed RDH8 supplies most reduction of bleach-generated all-trans-retinal in mouse rod outer segments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"6c52da43-9306-53e1-9429-363195bd7f3c","mechanism_event_label":"RDH8 converts the aldehyde photoproduct back to retinol in the outer segment.","subject":{"id":"91c82b57-8530-5b18-874d-002f1f610c6f","slug":"rdh8","display_name":"Retinol dehydrogenase 8 / RDH8","entity_type_key":"protein"},"object":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"6c52da43-9306-53e1-9429-363195bd7f3c","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:a-vision-rdh8-reduction-event","event_type":"biochemical_relationship","label":"RDH8 converts the aldehyde photoproduct back to retinol in the outer segment.","description":"Single-cell knockout comparisons showed RDH8 supplies most reduction of bleach-generated all-trans-retinal in mouse rod outer segments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"194eccf2-c7a5-52a6-a0c6-1c1f6d0f3a3e","slug":"all-trans-retinal","display_name":"All-trans-retinal","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"91c82b57-8530-5b18-874d-002f1f610c6f","slug":"rdh8","display_name":"Retinol dehydrogenase 8 / RDH8","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Wild-type/Rdh8-null/Rdh12-null rod fluorescence","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Residual reductases exist; this does not establish an exclusive enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"RDH8 converts the aldehyde photoproduct back to retinol in the outer segment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chen-2012] Reduction of all-trans-retinal in vertebrate rod photoreceptors requires the combined action of RDH8 and RDH12 (2012). https://pubmed.ncbi.nlm.nih.gov/22621924/ DOI: 10.1074/jbc.M112.354514","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Rod outer segment","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"66c778a4-367b-57f8-872f-5f66a1e1c3f5","evidence_kind":"source_excerpt","locator":"Lines 756-765","start_line":756,"end_line":765,"excerpt":"### a-vision-rdh8-reduction\nSingle-cell knockout comparisons showed RDH8 supplies most reduction of bleach-generated all-trans-retinal in mouse rod outer segments.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: RDH8 converts the aldehyde photoproduct back to retinol in the outer segment.\norganism: Mus musculus\ntissue_or_cell_type: Rod outer segment\nexperimental_model: Wild-type/Rdh8-null/Rdh12-null rod fluorescence\nlimitations: Residual reductases exist; this does not establish an exclusive enzyme.\n[chen-2012] Reduction of all-trans-retinal in vertebrate rod photoreceptors requires the combined action of RDH8 and RDH12 (2012). https://pubmed.ncbi.nlm.nih.gov/22621924/ DOI: 10.1074/jbc.M112.354514","model_system":"Wild-type/Rdh8-null/Rdh12-null rod fluorescence","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [chen-2012] Reduction of all-trans-retinal in vertebrate rod photoreceptors requires the combined action of RDH8 and RDH12 (2012). https://pubmed.ncbi.nlm.nih.gov/22621924/ DOI: 10.1074/jbc.M112.354514","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}