{"id":"4bc7ab03-c276-5de3-a2ef-79eb53b84e66","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-cystine-reduction-rescue","predicate":"reduces","statement":"Preventing cystine uptake or removing cystine relieved the disulfide stress associated with glucose deprivation in SLC7A11-high cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8069b225-9ca2-5b2e-9f81-4fc879764801","mechanism_event_label":"Reducing the incoming load helped when the cell could not process it.","subject":{"id":"ed488aae-8546-56b4-bcd2-3e4c4c0eabb4","slug":"human-cystine-removal-glucose-starvation","display_name":"Cystine removal during glucose starvation","entity_type_key":"cellular_process"},"object":{"id":"796fe565-258e-5f25-8057-2772a29294a9","slug":"human-slc7a11-high-disulfide-stress","display_name":"Disulfide stress in SLC7A11-high human cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8069b225-9ca2-5b2e-9f81-4fc879764801","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-cystine-reduction-rescue-event","event_type":"observed_relationship","label":"Reducing the incoming load helped when the cell could not process it.","description":"Preventing cystine uptake or removing cystine relieved the disulfide stress associated with glucose deprivation in SLC7A11-high cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ed488aae-8546-56b4-bcd2-3e4c4c0eabb4","slug":"human-cystine-removal-glucose-starvation","display_name":"Cystine removal during glucose starvation","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"796fe565-258e-5f25-8057-2772a29294a9","slug":"human-slc7a11-high-disulfide-stress","display_name":"Disulfide stress in SLC7A11-high human cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"aac19cc3-1b68-5ca3-a896-f61184f7320a","slug":"slc7a11","display_name":"SLC7A11","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"cdd84f4d-3e8f-502a-a8c5-19672cc5346f","slug":"cystine","display_name":"Cystine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cancer-cell glucose/cystine manipulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is distinct from cystine-withdrawal ferroptosis under other conditions; no universal benefit of removing cystine is implied.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Cysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Reducing the incoming load helped when the cell could not process it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cystine transporter regulation of pentose phosphate pathway dependency and disulfide stress exposes a targetable metabolic vulnerability in cancer. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32231310/ · DOI 10.1038/s41556-020-0496-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2e56627a-1179-5729-a2e1-c975940c6284","evidence_kind":"source_excerpt","locator":"Lines 244-250","start_line":244,"end_line":250,"excerpt":"## l-cysteine-cystine-reduction-rescue\nReducing the incoming load helped when the cell could not process it.\nPreventing cystine uptake or removing cystine relieved the disulfide stress associated with glucose deprivation in SLC7A11-high cells.\nModel: Human cancer-cell glucose/cystine manipulation.\nLimitations: This is distinct from cystine-withdrawal ferroptosis under other conditions; no universal benefit of removing cystine is implied.\nEvidence access: Primary full text\nCystine transporter regulation of pentose phosphate pathway dependency and disulfide stress exposes a targetable metabolic vulnerability in cancer. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32231310/ · DOI 10.1038/s41556-020-0496-x","model_system":"Human cancer-cell glucose/cystine manipulation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"654560f6-8d7f-596b-8722-48d94053cfe3","stable_key":"import-a8baf7e9-80e4-5d8c-adec-9a63e84d2f21","title":"L-Cysteine: sulfur allocation, redox supply and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f722669e54eab08ffe7289f9d79ddfc443014ed8c4bc5ba10ce2635470d22498","revision_id":"8ae25609-a031-5782-9c62-6cad8767ea46","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}