{"id":"4b89a36e-9d87-5f41-8719-6dc89b1b76ef","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:mthfd1-s-phase-localization","predicate":"localizes-to","statement":"MTHFD1 accumulated in nuclei of S-phase HeLa and MCF7 cells.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b7d39407-d275-54dd-897c-ff136c5061da","mechanism_event_label":"The enzyme moves toward the compartment where DNA is copied.","subject":{"id":"ae1cf62b-2ef9-52d7-bbf6-6817abb50d98","slug":"mthfd1","display_name":"Human MTHFD1","entity_type_key":"protein"},"object":{"id":"92a7a88e-c4dc-5d6d-babc-696ef95fff4b","slug":"nucleus","display_name":"Nucleus","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"b7d39407-d275-54dd-897c-ff136c5061da","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:mthfd1-s-phase-localization-event","event_type":"observed_intervention","label":"The enzyme moves toward the compartment where DNA is copied.","description":"MTHFD1 accumulated in nuclei of S-phase HeLa and MCF7 cells.","status":"provisional","compartment":{"slug":"nucleus","display_name":"Nucleus"},"participants":[{"entity":{"id":"ae1cf62b-2ef9-52d7-bbf6-6817abb50d98","slug":"mthfd1","display_name":"Human MTHFD1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"92a7a88e-c4dc-5d6d-babc-696ef95fff4b","slug":"nucleus","display_name":"Nucleus","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Cell-cycle localization experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cultured-cell localization; not a whole-body folate biomarker.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The enzyme moves toward the compartment where DNA is copied.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[field-2014] Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency (2014). https://pubmed.ncbi.nlm.nih.gov/25213861/ DOI: 10.1074/jbc.m114.599589","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HeLa and MCF7 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a1c6e5eb-40fb-5482-a9e4-907743d8b709","evidence_kind":"source_excerpt","locator":"Lines 949-959","start_line":949,"end_line":959,"excerpt":"### mthfd1-s-phase-localization\nMTHFD1 accumulated in nuclei of S-phase HeLa and MCF7 cells.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The enzyme moves toward the compartment where DNA is copied.\norganism: Homo sapiens\ntissue_or_cell_type: HeLa and MCF7 cells\nexperimental_model: Cell-cycle localization experiments\nlimitations: Cultured-cell localization; not a whole-body folate biomarker.\nexposure: Assay conditions described in the linked primary study.\n[field-2014] Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency (2014). https://pubmed.ncbi.nlm.nih.gov/25213861/ DOI: 10.1074/jbc.m114.599589","model_system":"Cell-cycle localization experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [field-2014] Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency (2014). https://pubmed.ncbi.nlm.nih.gov/25213861/ DOI: 10.1074/jbc.m114.599589","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}