{"id":"4b2a7e70-82e3-5d15-b7aa-ee589b70e9e8","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-young-dna-trend","predicate":"depletion_has_uncertain_effect_on","statement":"Seven weeks at 115 micrograms DFE/day produced a nonsignificant tendency toward increased DNA methyl acceptance (P=0.08).","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0395c0db-a580-529c-a679-1ffd2e471f9c","mechanism_event_label":"This result suggested but did not establish a methylation decrease.","subject":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"object":{"id":"a28c2ffa-8f0b-530a-91e0-770c661a3583","slug":"genomic-dna-methylation","display_name":"Genomic DNA methylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0395c0db-a580-529c-a679-1ffd2e471f9c","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-young-dna-trend-event","event_type":"observed_intervention","label":"This result suggested but did not establish a methylation decrease.","description":"Seven weeks at 115 micrograms DFE/day produced a nonsignificant tendency toward increased DNA methyl acceptance (P=0.08).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a28c2ffa-8f0b-530a-91e0-770c661a3583","slug":"genomic-dna-methylation","display_name":"Genomic DNA methylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Young MTHFR 677CC/TT women; controlled folate feeding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay-specific trend, not demonstrated global hypomethylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This result suggested but did not establish a methylation decrease.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[shelnutt-2004] Methylenetetrahydrofolate reductase 677C-->T polymorphism affects DNA methylation in response to controlled folate intake in young women (2004). https://pubmed.ncbi.nlm.nih.gov/15350988/ DOI: 10.1016/j.jnutbio.2004.04.003","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Blood-derived genomic DNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"9b293779-7542-5827-a815-7626facf3715","evidence_kind":"source_excerpt","locator":"Lines 789-798","start_line":789,"end_line":798,"excerpt":"### folate-methyl-young-dna-trend\nSeven weeks at 115 micrograms DFE/day produced a nonsignificant tendency toward increased DNA methyl acceptance (P=0.08).\nCondition category: nutrient_deficiency\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This result suggested but did not establish a methylation decrease.\norganism: Homo sapiens\ntissue_or_cell_type: Blood-derived genomic DNA\nexperimental_model: Young MTHFR 677CC/TT women; controlled folate feeding.\nlimitations: Assay-specific trend, not demonstrated global hypomethylation.\n[shelnutt-2004] Methylenetetrahydrofolate reductase 677C-->T polymorphism affects DNA methylation in response to controlled folate intake in young women (2004). https://pubmed.ncbi.nlm.nih.gov/15350988/ DOI: 10.1016/j.jnutbio.2004.04.003","model_system":"Young MTHFR 677CC/TT women; controlled folate feeding.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [shelnutt-2004] Methylenetetrahydrofolate reductase 677C-->T polymorphism affects DNA methylation in response to controlled folate intake in young women (2004). https://pubmed.ncbi.nlm.nih.gov/15350988/ DOI: 10.1016/j.jnutbio.2004.04.003","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}