{"id":"4af9cbed-0b85-5f1a-898d-ed9c8f0ba646","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-human-tdh-boundary","predicate":"does_not_supply","statement":"Human TDH transcripts encode truncated proteins because of splice-site disruption and a premature stop codon; the gene was classified as an expressed pseudogene.","claim_class":"identity","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"1a1b5951-37a2-5b35-9e3f-04d9c51f1130","mechanism_event_label":"An expressed gene is not necessarily a functioning metabolic route.","subject":{"id":"4d6712b8-f9ac-5969-9420-659d2ef11a23","slug":"human-tdh-pseudogene","display_name":"Human TDH expressed pseudogene","entity_type_key":"gene"},"object":{"id":"807658a1-4d50-57d0-b61c-157b814a3063","slug":"human-functional-tdh-route","display_name":"Functional canonical human TDH threonine-oxidation route","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1a1b5951-37a2-5b35-9e3f-04d9c51f1130","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-human-tdh-boundary-event","event_type":"observed_relationship","label":"An expressed gene is not necessarily a functioning metabolic route.","description":"Human TDH transcripts encode truncated proteins because of splice-site disruption and a premature stop codon; the gene was classified as an expressed pseudogene.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4d6712b8-f9ac-5969-9420-659d2ef11a23","slug":"human-tdh-pseudogene","display_name":"Human TDH expressed pseudogene","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"807658a1-4d50-57d0-b61c-157b814a3063","slug":"human-functional-tdh-route","display_name":"Functional canonical human TDH threonine-oxidation route","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cDNA/genomic analysis; exon-6 splice-site loss in all 23 genotyped individuals.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not transfer intact mouse Tdh-dependent glycine and acetyl-CoA synthesis to humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An expressed gene is not necessarily a functioning metabolic route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The human L-threonine 3-dehydrogenase gene is an expressed pseudogene. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12361482/ · DOI 10.1186/1471-2156-3-18","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"41800bc3-43a0-57a1-9fa9-003215f114b6","evidence_kind":"source_excerpt","locator":"Lines 274-280","start_line":274,"end_line":280,"excerpt":"## l-threonine-human-tdh-boundary\nAn expressed gene is not necessarily a functioning metabolic route.\nHuman TDH transcripts encode truncated proteins because of splice-site disruption and a premature stop codon; the gene was classified as an expressed pseudogene.\nModel: Human cDNA/genomic analysis; exon-6 splice-site loss in all 23 genotyped individuals.\nLimitations: Do not transfer intact mouse Tdh-dependent glycine and acetyl-CoA synthesis to humans.\nEvidence access: Primary abstract\nThe human L-threonine 3-dehydrogenase gene is an expressed pseudogene. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12361482/ · DOI 10.1186/1471-2156-3-18","model_system":"Human cDNA/genomic analysis; exon-6 splice-site loss in all 23 genotyped individuals.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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