{"id":"4af10bb9-183c-5bb6-a1fc-71e31b492618","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-wnt5a-expression","predicate":"increases_expression","statement":"Strontium ranelate increased WNT5A expression in the murine osteoblast study; calcineurin inhibitors abolished the increase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"40104073-77d1-5bbd-862b-7cedd761eb0e","mechanism_event_label":"One signaling response changes the production of another signaling protein.","subject":{"id":"a319eca8-b0ed-5a8c-8659-e7f106bed73a","slug":"strontium-ranelate","display_name":"Strontium ranelate","entity_type_key":"chemical_species"},"object":{"id":"7e74ffe9-57d9-5504-98a8-5f5e6363159b","slug":"mouse-wnt5a","display_name":"Mouse Wnt5a","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"40104073-77d1-5bbd-862b-7cedd761eb0e","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-wnt5a-expression-event","event_type":"observed_relationship","label":"One signaling response changes the production of another signaling protein.","description":"Strontium ranelate increased WNT5A expression in the murine osteoblast study; calcineurin inhibitors abolished the increase.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a319eca8-b0ed-5a8c-8659-e7f106bed73a","slug":"strontium-ranelate","display_name":"Strontium ranelate","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7e74ffe9-57d9-5504-98a8-5f5e6363159b","slug":"mouse-wnt5a","display_name":"Mouse Wnt5a","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2ca2864b-1ef0-54e4-8397-1dbe7207a0d2","slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4c1eda36-bce1-580b-b308-7cdff624f780","slug":"mouse-calcineurin","display_name":"Mouse calcineurin phosphatase complex","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b4a1bee0-582b-5c47-817f-04b6764cfbe8","slug":"mouse-nfatc1","display_name":"Mouse NFATc1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Murine osteoblast gene-expression experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression is not equivalent to ligand delivery in human bone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"One signaling response changes the production of another signaling protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Essential role of nuclear factor of activated T cells (NFAT)-mediated Wnt signaling in osteoblast differentiation induced by strontium ranelate. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20554534/ · DOI 10.1074/jbc.M110.110502","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2e436b92-f470-5e7b-8d94-1d241ef018c6","evidence_kind":"source_excerpt","locator":"Lines 142-148","start_line":142,"end_line":148,"excerpt":"## strontium-wnt5a-expression\nOne signaling response changes the production of another signaling protein.\nStrontium ranelate increased WNT5A expression in the murine osteoblast study; calcineurin inhibitors abolished the increase.\nModel: Murine osteoblast gene-expression experiments.\nLimitations: Expression is not equivalent to ligand delivery in human bone.\nEvidence access: Primary abstract\nEssential role of nuclear factor of activated T cells (NFAT)-mediated Wnt signaling in osteoblast differentiation induced by strontium ranelate. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20554534/ · DOI 10.1074/jbc.M110.110502","model_system":"Murine osteoblast gene-expression experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"43ef0fe7-eb6c-5346-8381-f7694733b4dc","stable_key":"import-1ff3a29f-ef80-5bff-9300-30b98c60b90d","title":"Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"51221949263cdf8a22a79c32e3989599de1a37dc215700e0a076f436b40a1005","revision_id":"84f6df49-c456-5ad2-8214-cc69d5dc7722","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}