{"id":"4ad1a2fa-e9d4-50b9-9da2-a8eee3cf7982","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-vrac-volume","predicate":"supports","statement":"Regulatory cell-volume decrease depended on LRRC8 proteins.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"72cb8fb9-6530-53aa-9108-8936032610ca","mechanism_event_label":"Cells use anion movement as part of their response to swelling.","subject":{"id":"efa2cfaf-c73a-5d4e-a623-0c4c0d93966d","slug":"lrrc8-vrac","display_name":"LRRC8 heteromeric volume-regulated anion channel / VRAC","entity_type_key":"protein_complex"},"object":{"id":"d4420b0e-04a2-58bb-9875-d74befdd0ebc","slug":"regulatory-volume-decrease","display_name":"Regulatory cell-volume decrease","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"72cb8fb9-6530-53aa-9108-8936032610ca","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-vrac-volume-event","event_type":"biochemical_relationship","label":"Cells use anion movement as part of their response to swelling.","description":"Regulatory cell-volume decrease depended on LRRC8 proteins.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"efa2cfaf-c73a-5d4e-a623-0c4c0d93966d","slug":"lrrc8-vrac","display_name":"LRRC8 heteromeric volume-regulated anion channel / VRAC","entity_type_key":"protein_complex"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d4420b0e-04a2-58bb-9875-d74befdd0ebc","slug":"regulatory-volume-decrease","display_name":"Regulatory cell-volume decrease","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/24790029.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9fc0ba50276b74ac5cd7aa29c4c8af4917132a43259b45363b5c16a19b775334\", \"start_char\": 0, \"end_char\": 896, \"text_sha256\": \"9fc0ba50276b74ac5cd7aa29c4c8af4917132a43259b45363b5c16a19b775334\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Genome-wide screen, gene disruption and rescue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"LRRC8 knockout and isoform coexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Channel composition and cellular volume response; no dietary intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human cell lines","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Cells use anion movement as part of their response to swelling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p24790029] Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. (2014). https://pubmed.ncbi.nlm.nih.gov/24790029/ DOI: 10.1126/science.1252826","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c407ad93-d679-5f37-8176-b42619214d33","evidence_kind":"source_excerpt","locator":"Lines 484-495","start_line":484,"end_line":495,"excerpt":"### chloride-vrac-volume\nRegulatory cell-volume decrease depended on LRRC8 proteins.\nCondition category: normal\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Cells use anion movement as part of their response to swelling.\norganism: Human cell lines\ntissue_or_cell_type: Plasma membrane\nexperimental_model: Genome-wide screen, gene disruption and rescue\nlimitations: Channel composition and cellular volume response; no dietary intervention.\nexposure: LRRC8 knockout and isoform coexpression\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/24790029.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9fc0ba50276b74ac5cd7aa29c4c8af4917132a43259b45363b5c16a19b775334\", \"start_char\": 0, \"end_char\": 896, \"text_sha256\": \"9fc0ba50276b74ac5cd7aa29c4c8af4917132a43259b45363b5c16a19b775334\"}\n[chloride-p24790029] Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. 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