{"id":"4a9615ab-03f1-54ec-b747-a5c9e12bd51f","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:mmaa-protects-mmut-activity","predicate":"protects-catalytic-activity-of","statement":"Adding human MMAA at reaction initiation protected recombinant human MMUT against catalytic inactivation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5a4e6237-180e-5605-bc27-a2035505d4ea","mechanism_event_label":"MMAA helped purified human MMUT remain active during the tested reaction.","subject":{"id":"39366dce-a30d-5e74-8c86-2bd8ca0bad96","slug":"mmaa","display_name":"Human MMAA GTPase","entity_type_key":"protein"},"object":{"id":"8f690797-c976-5a5e-886e-dc49d1b17f5a","slug":"mmut","display_name":"Human methylmalonyl-CoA mutase / MMUT","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"5a4e6237-180e-5605-bc27-a2035505d4ea","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:mmaa-protects-mmut-activity-event","event_type":"biochemical_relationship","label":"MMAA helped purified human MMUT remain active during the tested reaction.","description":"Adding human MMAA at reaction initiation protected recombinant human MMUT against catalytic inactivation.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"07657685-74aa-5d99-8625-0f6a3b521f07","slug":"adenosylcobalamin","display_name":"5'-Deoxyadenosylcobalamin","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"39366dce-a30d-5e74-8c86-2bd8ca0bad96","slug":"mmaa","display_name":"Human MMAA GTPase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8f690797-c976-5a5e-886e-dc49d1b17f5a","slug":"mmut","display_name":"Human methylmalonyl-CoA mutase / MMUT","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Indexed primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"MMAA added at reaction initiation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Biochemical reconstitution; does not determine cellular rate or dietary requirement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"MMAA helped purified human MMUT remain active during the tested reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[takahashi-2010-mmaa-repair] Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein. (2011). https://pubmed.ncbi.nlm.nih.gov/21138732/ DOI: 10.1016/j.bbrc.2010.11.141","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified protein assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2bd608af-1dab-59c4-8376-3394e2e6a2da","evidence_kind":"source_excerpt","locator":"Lines 1075-1087","start_line":1075,"end_line":1087,"excerpt":"### mmaa-protects-mmut-activity\nAdding human MMAA at reaction initiation protected recombinant human MMUT against catalytic inactivation.\nCondition category: normal\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: MMAA helped purified human MMUT remain active during the tested reaction.\norganism: Homo sapiens\ntissue_or_cell_type: Purified protein assay\nexperimental_model: Purified human proteins\nlimitations: Biochemical reconstitution; does not determine cellular rate or dietary requirement.\nexposure: MMAA added at reaction initiation\ncross_nutrient: false\nevidence_location: Indexed primary abstract\n[takahashi-2010-mmaa-repair] Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein. (2011). https://pubmed.ncbi.nlm.nih.gov/21138732/ DOI: 10.1016/j.bbrc.2010.11.141","model_system":"Purified human proteins","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [takahashi-2010-mmaa-repair] Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein. (2011). https://pubmed.ncbi.nlm.nih.gov/21138732/ DOI: 10.1016/j.bbrc.2010.11.141","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73145023-2982-5848-aff2-8d8875d6b9c5","stable_key":"import-7fc92b9e-9cbf-556e-8719-6b5244625250","title":"Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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