{"id":"4a1dcafd-5695-505d-ac16-0b5cec61ea4a","stable_key":"e1af6d55-ced5-56c9-9a2d-8cc2ae6ec3ff:nasunin-endothelial-tube-null","predicate":"did_not_significantly_change","statement":"Nasunin did not significantly change HUVEC tube formation on reconstituted basement membrane.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"186bc170-c6f3-5ab0-8fe4-17ec508af927","mechanism_event_label":"The tube-formation endpoint was also negative.","subject":{"id":"6553997f-f098-5507-95b4-0fdb8aac491d","slug":"nasunin","display_name":"Nasunin","entity_type_key":"small_molecule"},"object":{"id":"2b020120-70b2-537a-b482-d760a83258a2","slug":"huvec-tube-formation","display_name":"Human umbilical vein endothelial cell tube formation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"186bc170-c6f3-5ab0-8fe4-17ec508af927","stable_key":"e1af6d55-ced5-56c9-9a2d-8cc2ae6ec3ff:nasunin-endothelial-tube-null-event","event_type":"biochemical_relationship","label":"The tube-formation endpoint was also negative.","description":"Nasunin did not significantly change HUVEC tube formation on reconstituted basement membrane.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6553997f-f098-5507-95b4-0fdb8aac491d","slug":"nasunin","display_name":"Nasunin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b020120-70b2-537a-b482-d760a83258a2","slug":"huvec-tube-formation","display_name":"Human umbilical vein endothelial cell tube formation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/nasunin-research/16076105.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5b1a82636d590c80ec052a338bd3ebf58a1094004238cfda2c404e8ee98f5ad2\", \"start_char\": 0, \"end_char\": 785, \"text_sha256\": \"5b1a82636d590c80ec052a338bd3ebf58a1094004238cfda2c404e8ee98f5ad2\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Aortic-ring outgrowth and separate endothelial assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Outgrowth suppression at concentrations above 10 micromolar; proliferation 50-200 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ex vivo and cell endpoints do not establish cancer prevention or direct VEGF/VEGFR inhibition. Null migration and tube assays are distinct endpoints, not contradictions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Nasunin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nasunin","display_name":"Nasunin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rat aortic rings and human HUVEC cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The tube-formation endpoint was also negative.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[nasunin-p16076105] Antiangiogenic activity of nasunin, an antioxidant anthocyanin, in eggplant peels. (2005). https://pubmed.ncbi.nlm.nih.gov/16076105/ DOI: 10.1021/jf050796r","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Microvessel formation, proliferation, migration and tubes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1f1c4f73-e1aa-5ef7-9c18-b164a9112ec2","evidence_kind":"source_excerpt","locator":"Lines 835-846","start_line":835,"end_line":846,"excerpt":"### nasunin-endothelial-tube-null\nNasunin did not significantly change HUVEC tube formation on reconstituted basement membrane.\nCondition category: normal\nnutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The tube-formation endpoint was also negative.\norganism: Rat aortic rings and human HUVEC cultures\ntissue_or_cell_type: Microvessel formation, proliferation, migration and tubes\nexperimental_model: Aortic-ring outgrowth and separate endothelial assays\nlimitations: Ex vivo and cell endpoints do not establish cancer prevention or direct VEGF/VEGFR inhibition. Null migration and tube assays are distinct endpoints, not contradictions.\nexposure: Outgrowth suppression at concentrations above 10 micromolar; proliferation 50-200 micromolar\nevidence_span: {\"source_cache\": \"artifacts/nasunin-research/16076105.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5b1a82636d590c80ec052a338bd3ebf58a1094004238cfda2c404e8ee98f5ad2\", \"start_char\": 0, \"end_char\": 785, \"text_sha256\": \"5b1a82636d590c80ec052a338bd3ebf58a1094004238cfda2c404e8ee98f5ad2\"}\n[nasunin-p16076105] Antiangiogenic activity of nasunin, an antioxidant anthocyanin, in eggplant peels. (2005). https://pubmed.ncbi.nlm.nih.gov/16076105/ DOI: 10.1021/jf050796r","model_system":"Aortic-ring outgrowth and separate endothelial assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [nasunin-p16076105] Antiangiogenic activity of nasunin, an antioxidant anthocyanin, in eggplant peels. (2005). https://pubmed.ncbi.nlm.nih.gov/16076105/ DOI: 10.1021/jf050796r","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2c43df19-ccb0-58f1-b9a4-bc92cda6de5a","stable_key":"import-e1af6d55-ced5-56c9-9a2d-8cc2ae6ec3ff","title":"Nasunin: identity, redox chemistry and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"56552c208433294bf9a6c95513d981dbe4d00fd8336c121816d7b8bd6604d50e","revision_id":"b0296d49-32d7-52dc-a23c-5e862da0fba3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}