{"id":"49c92c0f-f20d-5d79-812d-13afca3e7369","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-ppara-binding","predicate":"binds","statement":"Surface-plasmon-resonance assays detected binding of astaxanthin to recombinant human PPAR-alpha ligand-binding domains.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"950614c8-ab9a-5a51-b697-7ea20cb98cfb","mechanism_event_label":"A purified receptor domain interacted directly with the molecule.","subject":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"object":{"id":"88e0c274-be64-5116-99dd-75ee184ec64c","slug":"ppara","display_name":"Human PPAR alpha / PPARA","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"950614c8-ab9a-5a51-b697-7ea20cb98cfb","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-ppara-binding-event","event_type":"observed_relationship","label":"A purified receptor domain interacted directly with the molecule.","description":"Surface-plasmon-resonance assays detected binding of astaxanthin to recombinant human PPAR-alpha ligand-binding domains.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"88e0c274-be64-5116-99dd-75ee184ec64c","slug":"ppara","display_name":"Human PPAR alpha / PPARA","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, methods 2.4 and SPR results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human receptor-domain biochemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Domain binding does not establish receptor occupancy after oral supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Astaxanthin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A purified receptor domain interacted directly with the molecule.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The natural carotenoid astaxanthin, a PPAR-α agonist and PPAR-γ antagonist, reduces hepatic lipid accumulation by rewiring the transcriptome in lipid-loaded hepatocytes. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22707263/ · DOI 10.1002/mnfr.201100798","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"10942817-dac2-58e7-9170-79f562530f15","evidence_kind":"source_excerpt","locator":"Lines 246-252","start_line":246,"end_line":252,"excerpt":"## astaxanthin-ppara-binding\nA purified receptor domain interacted directly with the molecule.\nSurface-plasmon-resonance assays detected binding of astaxanthin to recombinant human PPAR-alpha ligand-binding domains.\nModel: Recombinant human receptor-domain biochemistry.\nLimitations: Domain binding does not establish receptor occupancy after oral supplementation.\nEvidence access: Primary full text, methods 2.4 and SPR results\nThe natural carotenoid astaxanthin, a PPAR-α agonist and PPAR-γ antagonist, reduces hepatic lipid accumulation by rewiring the transcriptome in lipid-loaded hepatocytes. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22707263/ · DOI 10.1002/mnfr.201100798","model_system":"Recombinant human receptor-domain biochemistry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4fd91ea6-78b4-5bfe-93e3-686fbef9f678","stable_key":"import-a8bedee0-a740-5bbb-921e-bfd144c7b68c","title":"Astaxanthin: transport, membrane chemistry, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"98640e32f62e6c0387578165342ad21b3d2325701fcfdbb5c48886c783040e5e","revision_id":"a82757ce-1feb-582c-a5fc-f7de36308f1f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}