{"id":"49a0d8ce-a8d9-5679-9c01-9070a397c2bf","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-csad-plp","predicate":"cofactor_for","statement":"The purified human CSAD characterized in this study was a PLP-dependent enzyme, linking its decarboxylation chemistry to the active cofactor form of vitamin B6.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"168797d5-2286-5a5c-8488-3c38d91b93f9","mechanism_event_label":"CSAD needs the active B6 cofactor, not just its cysteine-derived substrate.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"a5ef2431-b1fa-57c9-a6ea-c877d4a1e3ec","slug":"csad","display_name":"Human cysteine sulfinic acid decarboxylase / CSAD","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"168797d5-2286-5a5c-8488-3c38d91b93f9","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-csad-plp-event","event_type":"observed_relationship","label":"CSAD needs the active B6 cofactor, not just its cysteine-derived substrate.","description":"The purified human CSAD characterized in this study was a PLP-dependent enzyme, linking its decarboxylation chemistry to the active cofactor form of vitamin B6.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a5ef2431-b1fa-57c9-a6ea-c877d4a1e3ec","slug":"csad","display_name":"Human cysteine sulfinic acid decarboxylase / CSAD","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"eeff5174-9aa8-5f34-b107-e826edde648c","slug":"hypotaurine","display_name":"Hypotaurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, enzyme characterization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human recombinant CSAD biochemical characterization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cofactor dependence does not establish that extra B6 increases taurine in a replete person.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"CSAD needs the active B6 cofactor, not just its cysteine-derived substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A Novel, Easy Assay Method for Human Cysteine Sulfinic Acid Decarboxylase. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34068845/ · DOI 10.3390/life11050438","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"648fa438-c419-509e-8b02-8182c57c52fc","evidence_kind":"source_excerpt","locator":"Lines 41-47","start_line":41,"end_line":47,"excerpt":"## taurine-csad-plp\nCSAD needs the active B6 cofactor, not just its cysteine-derived substrate.\nThe purified human CSAD characterized in this study was a PLP-dependent enzyme, linking its decarboxylation chemistry to the active cofactor form of vitamin B6.\nModel: Human recombinant CSAD biochemical characterization.\nLimitations: Cofactor dependence does not establish that extra B6 increases taurine in a replete person.\nEvidence access: Primary full text, enzyme characterization\nA Novel, Easy Assay Method for Human Cysteine Sulfinic Acid Decarboxylase. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34068845/ · DOI 10.3390/life11050438","model_system":"Human recombinant CSAD biochemical characterization.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c6cb77619a374d9b1b04830032fdd3b33d269d160df5baba3778fad5adf57db5","revision_id":"cc45c491-cc6a-5426-ab72-d7c6d26c3614","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}