{"id":"498e5b66-74d6-520e-91d3-4bab0572bd74","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-reactivation","predicate":"increases","statement":"The promoter changes accompanied gene mRNA re-expression; RARbeta and hMLH1 protein re-expression was detected.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"7e519012-c0a8-5565-8653-9676237e4347","mechanism_event_label":"The study also checked gene expression, beyond a methylation signal.","subject":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"object":{"id":"98697fb3-cc33-51d5-8426-d43d71ec5e45","slug":"methylation-silenced-gene-expression","display_name":"Expression of methylation-silenced genes in cancer cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7e519012-c0a8-5565-8653-9676237e4347","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-reactivation-event","event_type":"observed_relationship","label":"The study also checked gene expression, beyond a methylation signal.","description":"The promoter changes accompanied gene mRNA re-expression; RARbeta and hMLH1 protein re-expression was detected.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"98697fb3-cc33-51d5-8426-d43d71ec5e45","slug":"methylation-silenced-gene-expression","display_name":"Expression of methylation-silenced genes in cancer cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Cancer-cell expression assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Dietary exposure and human disease outcomes were not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The study also checked gene expression, beyond a methylation signal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Tea polyphenol (-)-epigallocatechin-3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines. · 2003 · https://pubmed.ncbi.nlm.nih.gov/14633667/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1e57dc6c-ba58-5597-8e06-86c195094bcb","evidence_kind":"source_excerpt","locator":"Lines 268-274","start_line":268,"end_line":274,"excerpt":"## egcg-reactivation\nThe study also checked gene expression, beyond a methylation signal.\nThe promoter changes accompanied gene mRNA re-expression; RARbeta and hMLH1 protein re-expression was detected.\nModel: Cancer-cell expression assays.\nLimitations: Dietary exposure and human disease outcomes were not established.\nEvidence access: primary abstract.\nTea polyphenol (-)-epigallocatechin-3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines. · 2003 · https://pubmed.ncbi.nlm.nih.gov/14633667/","model_system":"Cancer-cell expression assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b101c8d4d96ee03ff97eb1922c7bb0f96943295af473fdc3ef98b0dbd62fe8c7","revision_id":"e19bc5b2-ee3c-5f67-b5c5-9dc3b7d5f850","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}