{"id":"495676e7-a81f-54c9-b3cb-fc05cc09df1d","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-alternative-macrophage","predicate":"inhibition_impairs","statement":"Acute inhibition of hypusination impaired alternative macrophage activation while largely sparing glycolysis-associated classical activation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3a1a7343-c4fa-5078-b886-41a4933a803f","mechanism_event_label":"The same pathway affects immune states differently.","subject":{"id":"e1bf3e36-5f3d-5e65-b356-cee03e8ec175","slug":"mouse-dhps","display_name":"Mouse deoxyhypusine synthase / Dhps","entity_type_key":"protein"},"object":{"id":"35fd5138-27ca-5e7b-9603-f5e68f07a8ac","slug":"mouse-il4-macrophage-activation","display_name":"Mouse IL-4-associated macrophage activation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3a1a7343-c4fa-5078-b886-41a4933a803f","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-alternative-macrophage-event","event_type":"observed_relationship","label":"The same pathway affects immune states differently.","description":"Acute inhibition of hypusination impaired alternative macrophage activation while largely sparing glycolysis-associated classical activation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e1bf3e36-5f3d-5e65-b356-cee03e8ec175","slug":"mouse-dhps","display_name":"Mouse deoxyhypusine synthase / Dhps","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"35fd5138-27ca-5e7b-9603-f5e68f07a8ac","slug":"mouse-il4-macrophage-activation","display_name":"Mouse IL-4-associated macrophage activation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse macrophage in-vitro and in-vivo models.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Alternative activation is not universally beneficial or equivalent to lower inflammation in every disease.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same pathway affects immune states differently.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31130465/ · DOI 10.1016/j.cmet.2019.05.003","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"501bd4d6-f488-5946-8dc2-aa97e27101fc","evidence_kind":"source_excerpt","locator":"Lines 294-300","start_line":294,"end_line":300,"excerpt":"## spermidine-alternative-macrophage\nThe same pathway affects immune states differently.\nAcute inhibition of hypusination impaired alternative macrophage activation while largely sparing glycolysis-associated classical activation.\nModel: Mouse macrophage in-vitro and in-vivo models.\nLimitations: Alternative activation is not universally beneficial or equivalent to lower inflammation in every disease.\nEvidence access: Primary full text\nPolyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31130465/ · DOI 10.1016/j.cmet.2019.05.003","model_system":"Mouse macrophage in-vitro and in-vivo models.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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