{"id":"4916d76c-98af-5c04-bd77-b30c72d3165e","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-rat-hydroxylation","predicate":"reported_relationship","statement":"Rat microsomes formed luteolin, scutellarein and isoscutellarein from apigenin.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"a20533bb-cf83-54c6-85b8-863aaec1044a","mechanism_event_label":"Rat microsomes formed luteolin, scutellarein and isoscutellarein from apigenin.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"1c88d041-8309-5a75-b451-f2bd00e10a47","slug":"isoscutellarein","display_name":"Isoscutellarein / 8-hydroxyapigenin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a20533bb-cf83-54c6-85b8-863aaec1044a","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-rat-hydroxylation-event","event_type":"biochemical_relationship","label":"Rat microsomes formed luteolin, scutellarein and isoscutellarein from apigenin.","description":"### api-rat-hydroxylation\n\nRat microsomes formed luteolin, scutellarein and isoscutellarein from apigenin.\nThe intact perfused rat liver did not yield recovered phase-I products in the same report; it formed conjugates. Do not equate microsomal capability with exported in-vivo flux.\nModel and exposure: Rat microsomal phase-I metabolism.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Metabolism of apigenin by rat liver phase I and phase ii enzymes and by isolated perfused rat liver. | 2004 | https://pubmed.ncbi.nlm.nih.gov/14709621/ | https://doi.org/10.1124/dmd.32.1.58 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"recorded factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"1c88d041-8309-5a75-b451-f2bd00e10a47","slug":"isoscutellarein","display_name":"Isoscutellarein / 8-hydroxyapigenin","entity_type_key":"small_molecule"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"one product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"89a73524-6293-56a6-8caf-6ecef2bc7c29","slug":"scutellarein","display_name":"Scutellarein / 6-hydroxyapigenin","entity_type_key":"small_molecule"},"role":"one product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat microsomal phase-I metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Rat microsomes formed luteolin, scutellarein and isoscutellarein from apigenin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolism of apigenin by rat liver phase I and phase ii enzymes and by isolated perfused rat liver. | https://pubmed.ncbi.nlm.nih.gov/14709621/ | https://doi.org/10.1124/dmd.32.1.58","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-rat-hydroxylation; The intact perfused rat liver did not yield recovered phase-I products in the same report; it formed conjugates. Do not equate microsomal capability with exported in-vivo flux.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"790609c2-121f-5ba1-9331-8659f79de136","evidence_kind":"source_excerpt","locator":"Lines 114-120","start_line":114,"end_line":120,"excerpt":"### api-rat-hydroxylation\n\nRat microsomes formed luteolin, scutellarein and isoscutellarein from apigenin.\nThe intact perfused rat liver did not yield recovered phase-I products in the same report; it formed conjugates. Do not equate microsomal capability with exported in-vivo flux.\nModel and exposure: Rat microsomal phase-I metabolism.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Metabolism of apigenin by rat liver phase I and phase ii enzymes and by isolated perfused rat liver. | 2004 | https://pubmed.ncbi.nlm.nih.gov/14709621/ | https://doi.org/10.1124/dmd.32.1.58 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Rat microsomal phase-I metabolism.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}