{"id":"4910333c-095a-506a-9899-23e5063de27c","stable_key":"research:gpx3-supports-no-platelet-restraint","predicate":"supports-signaling","statement":"Redox and platelet measurements support GPX3 preservation of NO-mediated platelet restraint.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:supported_interpretation","direction":"positive","is_public":true,"mechanism_event_id":"14812f44-482c-5d2c-8682-b01f88d42eb2","mechanism_event_label":"GPX3 may help preserve nitric oxide's platelet-inhibitory signal.","subject":{"id":"32f7aa34-135f-5bfb-9189-81634ad4a824","slug":"gpx3","display_name":"GPX3","entity_type_key":"protein"},"object":{"id":"f2a6b7ab-ad41-5ada-8895-41d6ce42f4e4","slug":"nitric-oxide","display_name":"NO","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"14812f44-482c-5d2c-8682-b01f88d42eb2","stable_key":"research:gpx3-supports-no-platelet-restraint","event_type":"mechanistic-interpretation","label":"GPX3 may help preserve nitric oxide's platelet-inhibitory signal.","description":"Redox and platelet measurements support GPX3 preservation of NO-mediated platelet restraint.","status":"active","compartment":{"slug":"extracellular-space","display_name":"Extracellular space"},"participants":[{"entity":{"id":"32f7aa34-135f-5bfb-9189-81634ad4a824","slug":"gpx3","display_name":"GPX3","entity_type_key":"protein"},"role":"protective-factor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f2a6b7ab-ad41-5ada-8895-41d6ce42f4e4","slug":"nitric-oxide","display_name":"NO","entity_type_key":"small_molecule"},"role":"signaling-molecule","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a83eed3d-6e9b-5730-b5aa-cc1448ee5337","slug":"platelet-activation","display_name":"Platelet activation","entity_type_key":"cellular_process"},"role":"restrained-process","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"plasma and platelets","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Gpx3 knockout","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Individual oxidant intermediates were not fully isolated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mouse","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a2855ca8-ed77-5dfc-8d90-08ab707f01bf","evidence_kind":"curated_literature_summary","locator":"lines 714-724","start_line":714,"end_line":724,"excerpt":"## gpx3-supports-no-platelet-restraint\n\nGPX3 may help preserve nitric oxide's platelet-inhibitory signal.\n\nRedox and platelet measurements support GPX3 preservation of NO-mediated platelet restraint.\n\nOrganism: mouse\nCell type: plasma and platelets\nExperimental model: Gpx3 knockout\nLimitations: Individual oxidant intermediates were not fully isolated.\nPrimary reference: [Glutathione Peroxidase-3 Deficiency Promotes Platelet-dependent Thrombosis in vivo](https://pmc.ncbi.nlm.nih.gov/articles/PMC3107543/)","model_system":"Gpx3 knockout","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"vascular-redox","plain_language":"GPX3 may help preserve nitric oxide's platelet-inhibitory signal.","evidence_scope":"supported_interpretation","papers":[{"key":"jin-2011-gpx3-platelets","title":"Glutathione Peroxidase-3 Deficiency Promotes Platelet-dependent Thrombosis in vivo","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC3107543/","doi":"10.1161/CIRCULATIONAHA.110.000034","year":2011,"model":"Gpx3-knockout mice and platelet/vascular assays","summary":"Finds platelet hyperreactivity and increased thrombosis, with evidence implicating altered redox and nitric-oxide signaling."}]}}