{"id":"490f8586-ac32-5151-a94c-abbae25648a3","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-nlrp3-ubiquitination","predicate":"increases_ubiquitination","statement":"Myricetin promoted ROS-independent NLRP3 ubiquitination in the reported inflammasome experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a2efae2a-90bf-59c0-af90-a3c687b01e3d","mechanism_event_label":"A protein modification can change assembly of an inflammatory complex.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"ac5ef071-80d2-521e-86fe-372eda174ffd","slug":"myricetin-nlrp3-assay","display_name":"NLRP3 in the myricetin ubiquitination assay; cell species unresolved in abstract","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a2efae2a-90bf-59c0-af90-a3c687b01e3d","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-nlrp3-ubiquitination-event","event_type":"observed_relationship","label":"A protein modification can change assembly of an inflammatory complex.","description":"Myricetin promoted ROS-independent NLRP3 ubiquitination in the reported inflammasome experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ac5ef071-80d2-521e-86fe-372eda174ffd","slug":"myricetin-nlrp3-assay","display_name":"NLRP3 in the myricetin ubiquitination assay; cell species unresolved in abstract","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cellular inflammasome experiments with mouse sepsis/peritonitis validation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell species and chain topology are unresolved in the accessed abstract; do not infer degradation or assign human protein identity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A protein modification can change assembly of an inflammatory complex.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Myricetin inhibits NLRP3 inflammasome activation via reduction of ROS-dependent ubiquitination of ASC and promotion of ROS-independent NLRP3 ubiquitination. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30594691/ · DOI 10.1016/j.taap.2018.12.019","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bfc0aef6-24b5-5ae6-a3fd-5f27520ab86a","evidence_kind":"source_excerpt","locator":"Lines 620-626","start_line":620,"end_line":626,"excerpt":"## myricetin-nlrp3-ubiquitination\nA protein modification can change assembly of an inflammatory complex.\nMyricetin promoted ROS-independent NLRP3 ubiquitination in the reported inflammasome experiment.\nModel: Cellular inflammasome experiments with mouse sepsis/peritonitis validation.\nLimitations: Cell species and chain topology are unresolved in the accessed abstract; do not infer degradation or assign human protein identity.\nEvidence access: Primary abstract\nMyricetin inhibits NLRP3 inflammasome activation via reduction of ROS-dependent ubiquitination of ASC and promotion of ROS-independent NLRP3 ubiquitination. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30594691/ · DOI 10.1016/j.taap.2018.12.019","model_system":"Cellular inflammasome experiments with mouse sepsis/peritonitis validation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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