{"id":"490aa65e-2f6b-5864-bdff-d6e6d35caeca","stable_key":"research:selenoo-hydrolyzes-nad","predicate":"hydrolyzes","statement":"SELENOO catalyzes NAD+ hydrolysis to NMN and AMP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"neutral","is_public":true,"mechanism_event_id":"6e4acd16-b572-5004-95f2-3b834f6ef6b2","mechanism_event_label":"SELENOO can split NAD into two smaller molecules.","subject":{"id":"90e4a389-1062-5489-bae1-d49f337c98f0","slug":"selenoo","display_name":"SELENOO","entity_type_key":"protein"},"object":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"6e4acd16-b572-5004-95f2-3b834f6ef6b2","stable_key":"research:selenoo-hydrolyzes-nad","event_type":"catalysis","label":"SELENOO can split NAD into two smaller molecules.","description":"SELENOO catalyzes NAD+ hydrolysis to NMN and AMP.","status":"active","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"90e4a389-1062-5489-bae1-d49f337c98f0","slug":"selenoo","display_name":"SELENOO","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d31225b7-8e94-5857-9ea1-67ac794b1f15","slug":"water","display_name":"H2O","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d1c59139-db61-5fa0-a25d-40c3e625152e","slug":"nmn","display_name":"NMN","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"d47321ac-44bb-5a88-afdb-6435b57a91b9","slug":"amp","display_name":"AMP","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"experimental cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical and cellular assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Recent 2026 finding; no dietary-dose inference.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mammalian","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5c446a25-c630-5c79-a6cd-c9b62132d736","evidence_kind":"curated_literature_summary","locator":"lines 438-448","start_line":438,"end_line":448,"excerpt":"## selenoo-hydrolyzes-nad\n\nSELENOO can split NAD into two smaller molecules.\n\nSELENOO catalyzes NAD+ hydrolysis to NMN and AMP.\n\nOrganism: mammalian\nCell type: experimental cells\nExperimental model: Biochemical and cellular assays\nLimitations: Recent 2026 finding; no dietary-dose inference.\nPrimary reference: [NAD+ hydrolysis catalyzed by SelO is required for mitochondrial homeostasis](https://pubmed.ncbi.nlm.nih.gov/41806834/)","model_system":"Biochemical and cellular assays","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"mitochondrial-metabolism","plain_language":"SELENOO can split NAD into two smaller molecules.","evidence_scope":"direct_experimental","papers":[{"key":"jia-2026-selenoo-nad","title":"NAD+ hydrolysis catalyzed by SelO is required for mitochondrial homeostasis","url":"https://pubmed.ncbi.nlm.nih.gov/41806834/","doi":"10.1016/j.cell.2026.01.033","year":2026,"model":"Biochemistry and mammalian-cell experiments; additional bacterial work","summary":"Identifies manganese-dependent NAD cleavage by SELENOO and a requirement for its C-terminal Sec-containing region."}]}}