{"id":"48e70d58-8bfe-566d-aa9d-8fa6cc435e59","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-xo-inhibition","predicate":"competitively_inhibits","statement":"Luteolin competitively inhibited purified bovine xanthine oxidase without time-dependent inhibition.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"725ce6aa-c80c-5cd3-9958-dde37edfd793","mechanism_event_label":"It interfered with the purine-breakdown enzyme.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"8f0bfaa1-4efc-586c-bbf9-764de45f4d91","slug":"bovine-xdh","display_name":"Bovine xanthine oxidoreductase / XDH","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"725ce6aa-c80c-5cd3-9958-dde37edfd793","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-xo-inhibition-event","event_type":"observed_relationship","label":"It interfered with the purine-breakdown enzyme.","description":"Luteolin competitively inhibited purified bovine xanthine oxidase without time-dependent inhibition.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8f0bfaa1-4efc-586c-bbf9-764de45f4d91","slug":"bovine-xdh","display_name":"Bovine xanthine oxidoreductase / XDH","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0a8b817b-52c5-5b98-b1d8-d6677651744c","slug":"xanthine","display_name":"Xanthine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"99343398-bc89-52c4-b2f7-a0f6e3869a57","slug":"hypoxanthine","display_name":"Hypoxanthine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified bovine enzyme steady-state kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Bovine enzyme evidence; not a clinical urate-lowering trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"It interfered with the purine-breakdown enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition studies of bovine xanthine oxidase by luteolin, silibinin, quercetin, and curcumin. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19388706/ · DOI 10.1021/np8007123","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ea79f7d4-68f2-51c2-a538-b7fa41ddc389","evidence_kind":"source_excerpt","locator":"Lines 284-290","start_line":284,"end_line":290,"excerpt":"## luteolin-xo-inhibition\nIt interfered with the purine-breakdown enzyme.\nLuteolin competitively inhibited purified bovine xanthine oxidase without time-dependent inhibition.\nModel: Purified bovine enzyme steady-state kinetics.\nLimitations: Bovine enzyme evidence; not a clinical urate-lowering trial.\nEvidence access: Primary abstract\nInhibition studies of bovine xanthine oxidase by luteolin, silibinin, quercetin, and curcumin. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19388706/ · DOI 10.1021/np8007123","model_system":"Purified bovine enzyme steady-state kinetics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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