{"id":"48e22b68-3d8f-5062-9da0-5f2eaeb6a8ff","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-icam1-downregulation","predicate":"reduces","statement":"Induction of ICAM-1 required simultaneous hypoxia and hypoglycaemia, and hyperbaric oxygen reduced its expression to control levels.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0a8c487b-c33a-5222-9628-ebb6520718d9","mechanism_event_label":"The treatment switched off the docking molecule that inflamed vessels put out.","subject":{"id":"581fa746-cb84-571b-87a0-4d461263f660","slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"},"object":{"id":"b6dbeb4a-0b7a-5e80-b384-ae55fc5be8c1","slug":"icam1","display_name":"Intercellular adhesion molecule 1 / ICAM-1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"0a8c487b-c33a-5222-9628-ebb6520718d9","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-icam1-downregulation-event","event_type":"biochemical_relationship","label":"The treatment switched off the docking molecule that inflamed vessels put out.","description":"Induction of ICAM-1 required simultaneous hypoxia and hypoglycaemia, and hyperbaric oxygen reduced its expression to control levels.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15c60de7-1564-5006-bcf7-997a9f7e38a1","slug":"endothelial-cell","display_name":"Vascular endothelial cell","entity_type_key":"cell_type"},"role":"affected_cell","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"581fa746-cb84-571b-87a0-4d461263f660","slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b6dbeb4a-0b7a-5e80-b384-ae55fc5be8c1","slug":"icam1","display_name":"Intercellular adhesion molecule 1 / ICAM-1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/hbot-research/10666024.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9bb8781cab37af1af9f292e4611c19f273de986e86be681a357058d2cfb87ad8\", \"start_char\": 0, \"end_char\": 1182, \"text_sha256\": \"9bb8781cab37af1af9f292e4611c19f273de986e86be681a357058d2cfb87ad8\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human umbilical vein and bovine aortic endothelial cells in an in vitro ischaemia-reperfusion model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Hyperbaric oxygen after combined hypoxia and hypoglycaemia; L-NAME","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An in vitro model requiring both hypoxia and hypoglycaemia to induce the adhesion molecule. The nitric oxide synthase inhibitor arm supports the proposed route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Hyperbaric oxygen research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.","comparator":null,"unit":null,"notes":"","entity":{"slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Human and bovine cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The treatment switched off the docking molecule that inflamed vessels put out.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hbot-p10666024] Hyperbaric oxygen downregulates ICAM-1 expression induced by hypoxia and hypoglycemia: the role of NOS. (2000). https://pubmed.ncbi.nlm.nih.gov/10666024/ DOI: 10.1152/ajpcell.2000.278.2.c292","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Vascular endothelium","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d772f482-4f70-5f47-af4a-34b1b020ae0c","evidence_kind":"source_excerpt","locator":"Lines 712-723","start_line":712,"end_line":723,"excerpt":"### hbot-icam1-downregulation\nInduction of ICAM-1 required simultaneous hypoxia and hypoglycaemia, and hyperbaric oxygen reduced its expression to control levels.\nCondition category: normal\nnutrient_topic: Hyperbaric oxygen research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.\nplain_language: The treatment switched off the docking molecule that inflamed vessels put out.\norganism: Human and bovine cells\ntissue_or_cell_type: Vascular endothelium\nexperimental_model: Human umbilical vein and bovine aortic endothelial cells in an in vitro ischaemia-reperfusion model\nlimitations: An in vitro model requiring both hypoxia and hypoglycaemia to induce the adhesion molecule. The nitric oxide synthase inhibitor arm supports the proposed route.\nexposure: Hyperbaric oxygen after combined hypoxia and hypoglycaemia; L-NAME\nevidence_span: {\"source_cache\": \"artifacts/hbot-research/10666024.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9bb8781cab37af1af9f292e4611c19f273de986e86be681a357058d2cfb87ad8\", \"start_char\": 0, \"end_char\": 1182, \"text_sha256\": \"9bb8781cab37af1af9f292e4611c19f273de986e86be681a357058d2cfb87ad8\"}\n[hbot-p10666024] Hyperbaric oxygen downregulates ICAM-1 expression induced by hypoxia and hypoglycemia: the role of NOS. (2000). https://pubmed.ncbi.nlm.nih.gov/10666024/ DOI: 10.1152/ajpcell.2000.278.2.c292","model_system":"Human umbilical vein and bovine aortic endothelial cells in an in vitro ischaemia-reperfusion model","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hbot-p10666024] Hyperbaric oxygen downregulates ICAM-1 expression induced by hypoxia and hypoglycemia: the role of NOS. 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