{"id":"48e08621-8908-5c43-843e-3044c56ef588","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-human-glutaredoxin-dha","predicate":"catalyzes-reduction-of","statement":"Purified human placental glutaredoxin catalyzed GSH-dependent reduction of DHA to ascorbate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"04f1d1e9-f8d3-536d-9e1f-c5bf38d33bb2","mechanism_event_label":"Glutaredoxin can use glutathione to restore reduced vitamin C.","subject":{"id":"9693fa26-7353-5e19-8cb0-c03c4564afea","slug":"glrx","display_name":"Human glutaredoxin 1 / GLRX","entity_type_key":"protein"},"object":{"id":"e6658c2b-ccff-5122-80a5-2e153f12ba90","slug":"dehydroascorbic-acid","display_name":"Dehydroascorbic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"04f1d1e9-f8d3-536d-9e1f-c5bf38d33bb2","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-human-glutaredoxin-dha-event","event_type":"biochemical_relationship","label":"Glutaredoxin can use glutathione to restore reduced vitamin C.","description":"Purified human placental glutaredoxin catalyzed GSH-dependent reduction of DHA to ascorbate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"electron donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9693fa26-7353-5e19-8cb0-c03c4564afea","slug":"glrx","display_name":"Human glutaredoxin 1 / GLRX","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e6658c2b-ccff-5122-80a5-2e153f12ba90","slug":"dehydroascorbic-acid","display_name":"Dehydroascorbic acid","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human placental glutaredoxin and bovine liver PDI; additional porcine/bovine glutaredoxins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified enzyme plus DHA and GSH","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In vitro activity does not establish its share of total placental recycling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Glutaredoxin can use glutathione to restore reduced vitamin C.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[wells1990] Mammalian thioltransferase (glutaredoxin) and protein disulfide isomerase have dehydroascorbate reductase activity. (1990). https://pubmed.ncbi.nlm.nih.gov/2394726/ DOI: 10.1016/s0021-9258(18)55401-6","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Placental enzyme preparation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"94e11b4d-201e-584f-80e4-e2b0741ee69c","evidence_kind":"source_excerpt","locator":"Lines 351-362","start_line":351,"end_line":362,"excerpt":"### vc-transport-human-glutaredoxin-dha\nPurified human placental glutaredoxin catalyzed GSH-dependent reduction of DHA to ascorbate.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Glutaredoxin can use glutathione to restore reduced vitamin C.\norganism: Homo sapiens\ntissue_or_cell_type: Placental enzyme preparation\nexperimental_model: Purified human placental glutaredoxin and bovine liver PDI; additional porcine/bovine glutaredoxins\nlimitations: In vitro activity does not establish its share of total placental recycling.\nexposure: Purified enzyme plus DHA and GSH\ncross_nutrient: true\n[wells1990] Mammalian thioltransferase (glutaredoxin) and protein disulfide isomerase have dehydroascorbate reductase activity. (1990). https://pubmed.ncbi.nlm.nih.gov/2394726/ DOI: 10.1016/s0021-9258(18)55401-6","model_system":"Purified human placental glutaredoxin and bovine liver PDI; additional porcine/bovine glutaredoxins","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [wells1990] Mammalian thioltransferase (glutaredoxin) and protein disulfide isomerase have dehydroascorbate reductase activity. (1990). https://pubmed.ncbi.nlm.nih.gov/2394726/ DOI: 10.1016/s0021-9258(18)55401-6","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fa811221-13bd-5c10-adc1-eaf097c7703c","stable_key":"import-cb568d28-484a-5c2e-9fcc-2d780358e514","title":"Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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