{"id":"48c984fd-569b-5fc3-80a8-6235fdf1f36a","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-mocs1b-processing","predicate":"is_processed_to","statement":"MOCS1AB fusion precursors underwent mitochondrial import and proteolytic cleavage that released the matrix-localized MOCS1B domain.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"aab5efa2-2fb6-5d43-af58-c0472c6ec5f2","mechanism_event_label":"A precursor protein must be imported and cut to release MOCS1B.","subject":{"id":"883820cd-bbf4-5660-99ed-f35544694cb2","slug":"mocs1ab","display_name":"Human MOCS1AB fusion precursor","entity_type_key":"protein"},"object":{"id":"7810bb70-aa96-53f6-9b5a-0a5b737721da","slug":"mocs1b","display_name":"Human molybdenum cofactor synthesis protein MOCS1B","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"aab5efa2-2fb6-5d43-af58-c0472c6ec5f2","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-mocs1b-processing-event","event_type":"biochemical_relationship","label":"A precursor protein must be imported and cut to release MOCS1B.","description":"MOCS1AB fusion precursors underwent mitochondrial import and proteolytic cleavage that released the matrix-localized MOCS1B domain.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"883820cd-bbf4-5660-99ed-f35544694cb2","slug":"mocs1ab","display_name":"Human MOCS1AB fusion precursor","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7810bb70-aa96-53f6-9b5a-0a5b737721da","slug":"mocs1b","display_name":"Human molybdenum cofactor synthesis protein MOCS1B","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/31996372.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a88a2e2c4aae83293fa6f1860d10bb41b41c32d1ad79b0b04cd5dd3eb02865c7\", \"start_char\": 0, \"end_char\": 1846, \"text_sha256\": \"a88a2e2c4aae83293fa6f1860d10bb41b41c32d1ad79b0b04cd5dd3eb02865c7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Alternative splicing, fluorescence microscopy, fractionation and mitochondrial targeting of human MOCS1 proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Type I-III MOCS1 splice constructs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Localization and processing experiments; no dietary iron, SAM or molybdenum intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A precursor protein must be imported and cut to release MOCS1B.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p31996372] Alternative splicing of the bicistronic gene molybdenum cofactor synthesis 1 (MOCS1) uncovers a novel mitochondrial protein maturation mechanism. (2020). https://pubmed.ncbi.nlm.nih.gov/31996372/ DOI: 10.1074/jbc.ra119.010720","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Transfected human-cell localization and mitochondrial matrix","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6da80671-0e96-5443-978d-a9ff261dc063","evidence_kind":"source_excerpt","locator":"Lines 300-311","start_line":300,"end_line":311,"excerpt":"### mo-mocs1b-processing\nMOCS1AB fusion precursors underwent mitochondrial import and proteolytic cleavage that released the matrix-localized MOCS1B domain.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A precursor protein must be imported and cut to release MOCS1B.\norganism: Homo sapiens\ntissue_or_cell_type: Transfected human-cell localization and mitochondrial matrix\nexperimental_model: Alternative splicing, fluorescence microscopy, fractionation and mitochondrial targeting of human MOCS1 proteins\nlimitations: Localization and processing experiments; no dietary iron, SAM or molybdenum intervention.\nexposure: Type I-III MOCS1 splice constructs\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/31996372.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a88a2e2c4aae83293fa6f1860d10bb41b41c32d1ad79b0b04cd5dd3eb02865c7\", \"start_char\": 0, \"end_char\": 1846, \"text_sha256\": \"a88a2e2c4aae83293fa6f1860d10bb41b41c32d1ad79b0b04cd5dd3eb02865c7\"}\n[mo-p31996372] Alternative splicing of the bicistronic gene molybdenum cofactor synthesis 1 (MOCS1) uncovers a novel mitochondrial protein maturation mechanism. (2020). https://pubmed.ncbi.nlm.nih.gov/31996372/ DOI: 10.1074/jbc.ra119.010720","model_system":"Alternative splicing, fluorescence microscopy, fractionation and mitochondrial targeting of human MOCS1 proteins","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p31996372] Alternative splicing of the bicistronic gene molybdenum cofactor synthesis 1 (MOCS1) uncovers a novel mitochondrial protein maturation mechanism. (2020). https://pubmed.ncbi.nlm.nih.gov/31996372/ DOI: 10.1074/jbc.ra119.010720","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1aa6da60-8284-5252-8dd9-ac2250d5ced5","stable_key":"import-dc8975b1-95ff-5d9b-be17-a1c04610cca7","title":"Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"143147eb9ac7fe750bdd33bb50d7773bc0cd49cc94faf219aebce3c3d760dd8f","revision_id":"90c2239d-290b-5e16-815a-f94d30569ebd","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}