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(2004). https://pubmed.ncbi.nlm.nih.gov/15465040/ DOI: 10.1016/j.bbrc.2004.09.073","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"mitochondrial enzyme preparation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e735e82f-8566-5114-8835-d214bb67141d","evidence_kind":"source_excerpt","locator":"Lines 332-345","start_line":332,"end_line":345,"excerpt":"### vd-act-cyp27a1-d2-c24\nHuman CYP27A1 hydroxylated ergocalciferol at C24 in the reported D2 substrate analysis.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: CYP27A1 modifies D2 at carbon 24.\norganism: Homo sapiens protein\ntissue_or_cell_type: mitochondrial enzyme preparation\nexperimental_model: Recombinant human CYP27A1 product identification\nlimitations: D2 C24/C27 hydroxylation must not be mislabeled as C25 activation or assumed to be the main route in vivo.\nexposure: D2 incubation; concentration/time not reported in retrieved abstract.\ncross_nutrient: false\nevidence_location: Primary abstract, substrate product positions and kinetic comparison.\nnutrient: Vitamin D2 and D3\n[shinkyo2004] Metabolism of vitamin D by human microsomal CYP2R1. 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