{"id":"488d9787-30a2-5b2d-91ee-62623789c91a","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-liver-gpx4-e-rescue","predicate":"supports-survival-after","statement":"Maternal dietary supplementation with 500 IU/kg DL-alpha-tocopheryl acetate through gestation and nursing allowed Alb-Cre;Gpx4fl/fl pups to survive to weaning, whereas liver-specific Gpx4-null pups on standard chow died within 48 hours after birth.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"7ce7c264-4534-517a-a8c1-7c86f7df4115","mechanism_event_label":"Extra dietary vitamin E rescued early survival in mice lacking liver GPX4.","subject":{"id":"10597737-a327-5b5b-9b97-517734df10c4","slug":"all-rac-alpha-tocopheryl-acetate","display_name":"All-rac-alpha-tocopheryl acetate","entity_type_key":"small_molecule"},"object":{"id":"0f74f458-e4e0-512e-814d-2ec3de0b0be8","slug":"gpx4-hepatocyte-loss","display_name":"Hepatocyte-directed GPX4 loss","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"7ce7c264-4534-517a-a8c1-7c86f7df4115","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-liver-gpx4-e-rescue-event","event_type":"biochemical_relationship","label":"Extra dietary vitamin E rescued early survival in mice lacking liver GPX4.","description":"Maternal dietary supplementation with 500 IU/kg DL-alpha-tocopheryl acetate through gestation and nursing allowed Alb-Cre;Gpx4fl/fl pups to survive to weaning, whereas liver-specific Gpx4-null pups on standard chow died within 48 hours after birth.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e9bb13ee-f238-51e6-9101-78ddfd11465c","slug":"gpx4","display_name":"GPX4","entity_type_key":"protein"},"role":"deleted hepatocyte enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"10597737-a327-5b5b-9b97-517734df10c4","slug":"all-rac-alpha-tocopheryl-acetate","display_name":"All-rac-alpha-tocopheryl acetate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0f74f458-e4e0-512e-814d-2ec3de0b0be8","slug":"gpx4-hepatocyte-loss","display_name":"Hepatocyte-directed GPX4 loss","entity_type_key":"protein_state"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Results/Table 2; standard-chow comparison Table 1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Alb-Cre conditional Gpx4 deletion and maternal diet","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"500 IU/kg DL-alpha-tocopheryl acetate during gestation/lactation; weaning at 3 weeks.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Alb-Cre Gpx4 loss is machinery impairment, not selenium deficiency; mouse survival rescue does not establish human substitutability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Extra dietary vitamin E rescued early survival in mice lacking liver GPX4.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ver-carlson2016] Glutathione peroxidase 4 and vitamin E cooperatively prevent hepatocellular degeneration. 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