{"id":"483ef8a9-ee06-5c29-953e-31953479332e","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-citrin-calcium-site","predicate":"binds_regulatory_efhand2","statement":"Human citrin regulatory-domain structures resolved calcium at EF-hand 2; the other EF-hand motifs were not all calcium-binding sites.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"04cefe7b-3ad5-53b5-b8f2-c000edd088c4","mechanism_event_label":"Several similarly named structural motifs do not mean several equivalent calcium switches.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"5252ba8d-8e58-5fa5-abdb-7bc97ef85818","slug":"slc25a13","display_name":"Human citrin / SLC25A13","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"04cefe7b-3ad5-53b5-b8f2-c000edd088c4","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-citrin-calcium-site-event","event_type":"observed_relationship","label":"Several similarly named structural motifs do not mean several equivalent calcium switches.","description":"Human citrin regulatory-domain structures resolved calcium at EF-hand 2; the other EF-hand motifs were not all calcium-binding sites.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5252ba8d-8e58-5fa5-abdb-7bc97ef85818","slug":"slc25a13","display_name":"Human citrin / SLC25A13","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SLC25A13 regulatory-domain crystallography; calcium-bound and calcium-free structures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Domain structures do not define a dietary calcium threshold or a treatment for citrin deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Several similarly named structural motifs do not mean several equivalent calcium switches.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Calcium-induced conformational changes of the regulatory domain of human mitochondrial aspartate/glutamate carriers. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25410934/ · DOI 10.1038/ncomms6491","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f5882308-bd11-5578-9f6f-b5db9f9cf744","evidence_kind":"source_excerpt","locator":"Lines 98-104","start_line":98,"end_line":104,"excerpt":"## l-aspartate-citrin-calcium-site\nSeveral similarly named structural motifs do not mean several equivalent calcium switches.\nHuman citrin regulatory-domain structures resolved calcium at EF-hand 2; the other EF-hand motifs were not all calcium-binding sites.\nModel: Human SLC25A13 regulatory-domain crystallography; calcium-bound and calcium-free structures.\nLimitations: Domain structures do not define a dietary calcium threshold or a treatment for citrin deficiency.\nEvidence access: Primary full text\nCalcium-induced conformational changes of the regulatory domain of human mitochondrial aspartate/glutamate carriers. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25410934/ · DOI 10.1038/ncomms6491","model_system":"Human SLC25A13 regulatory-domain crystallography; calcium-bound and calcium-free structures.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"67970dcb-34e0-5b0a-8c86-d8c3cc183444","stable_key":"import-e37461ea-ea5d-5e2c-8091-138305f6dd70","title":"L-Aspartate: redox transfer, nitrogen partitioning and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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