{"id":"47d3c520-4e72-57df-b6b3-99e66c99715a","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-sodium-independent-system-l","predicate":"predominantly_sodium_independent_uptake_in","statement":"Theanine uptake was mostly sodium independent in the cell-line screen supporting system L transport.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"56da7419-b144-5684-b9a2-84ca1e904852","mechanism_event_label":"Sodium dependence varies with the transport system and cell model.","subject":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"object":{"id":"4087d289-f80f-5708-9050-1285d16c8a88","slug":"theanine-cellular-uptake","display_name":"Cellular L-theanine uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"56da7419-b144-5684-b9a2-84ca1e904852","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-sodium-independent-system-l-event","event_type":"biochemical_relationship","label":"Sodium dependence varies with the transport system and cell model.","description":"Theanine uptake was mostly sodium independent in the cell-line screen supporting system L transport.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"tested_ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4087d289-f80f-5708-9050-1285d16c8a88","slug":"theanine-cellular-uptake","display_name":"Cellular L-theanine uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/23221699.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0f79abd668759eccd12f86ec895af75dadf94f6412c29d51a2b6cfde712f9372\", \"start_char\": 0, \"end_char\": 1143, \"text_sha256\": \"0f79abd668759eccd12f86ec895af75dadf94f6412c29d51a2b6cfde712f9372\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell uptake and stable human-transporter expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Radiolabeled theanine with leucine/BCH competition and sodium replacement","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human transporter identity confirmed in the public primary PDF methods. Engineered cells do not prove which transporter dominates human blood-brain-barrier flux or a clinical meal interaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mammalian cell lines; human LAT1 and LAT2 expressed in mouse S2 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Sodium dependence varies with the transport system and cell model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p23221699] The involvement of L-type amino acid transporters in theanine transport. (2012). https://pubmed.ncbi.nlm.nih.gov/23221699/ DOI: 10.1271/bbb.120519","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"System L amino acid transport","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9b595051-68e1-5c74-8daf-17a633b53639","evidence_kind":"source_excerpt","locator":"Lines 211-222","start_line":211,"end_line":222,"excerpt":"### theanine-sodium-independent-system-l\nTheanine uptake was mostly sodium independent in the cell-line screen supporting system L transport.\nCondition category: normal\nnutrient_topic: L-Theanine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Sodium dependence varies with the transport system and cell model.\norganism: Mammalian cell lines; human LAT1 and LAT2 expressed in mouse S2 cells\ntissue_or_cell_type: System L amino acid transport\nexperimental_model: Cell uptake and stable human-transporter expression\nlimitations: Human transporter identity confirmed in the public primary PDF methods. Engineered cells do not prove which transporter dominates human blood-brain-barrier flux or a clinical meal interaction.\nexposure: Radiolabeled theanine with leucine/BCH competition and sodium replacement\nevidence_span: {\"source_cache\": \"artifacts/theanine-research/23221699.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0f79abd668759eccd12f86ec895af75dadf94f6412c29d51a2b6cfde712f9372\", \"start_char\": 0, \"end_char\": 1143, \"text_sha256\": \"0f79abd668759eccd12f86ec895af75dadf94f6412c29d51a2b6cfde712f9372\"}\n[theanine-p23221699] The involvement of L-type amino acid transporters in theanine transport. (2012). https://pubmed.ncbi.nlm.nih.gov/23221699/ DOI: 10.1271/bbb.120519","model_system":"Cell uptake and stable human-transporter expression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [theanine-p23221699] The involvement of L-type amino acid transporters in theanine transport. (2012). https://pubmed.ncbi.nlm.nih.gov/23221699/ DOI: 10.1271/bbb.120519","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2bf3fc8-fc9a-5d50-937a-559a76f8be7d","stable_key":"import-8f12ba85-1a92-5337-a9c0-9cee9b3be90d","title":"L-Theanine: metabolism, neural signaling, nutrient connections and human outcomes (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"c78f2a1961da546cd8d3ae9131a16613aed0d1032904ca19175b0641647142c1","revision_id":"4ec64995-1e5b-5279-9102-f203cb2e29e5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}