{"id":"47300e99-52ca-5804-94b3-c030faa0910d","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-proteasome-block","predicate":"inhibition_removes_protection","statement":"Proteasome inhibitors nearly abolished fisetin-supported survival of trophic-factor-deprived rat cortical neurons.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3c773a70-08ed-5cbb-b17b-3df51da44789","mechanism_event_label":"Blocking downstream machinery prevented the response.","subject":{"id":"6996fb6e-548d-597b-8a6b-97e4b468da41","slug":"rat-neuronal-proteasome","display_name":"Rat cortical-neuron proteasome activity","entity_type_key":"protein_complex"},"object":{"id":"9a63c015-5a54-561f-8e95-356c3ddad196","slug":"rat-cortical-neuron-survival-without-trophic-factors","display_name":"Rat cortical-neuron survival after trophic-factor withdrawal","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3c773a70-08ed-5cbb-b17b-3df51da44789","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-proteasome-block-event","event_type":"observed_relationship","label":"Blocking downstream machinery prevented the response.","description":"Proteasome inhibitors nearly abolished fisetin-supported survival of trophic-factor-deprived rat cortical neurons.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6996fb6e-548d-597b-8a6b-97e4b468da41","slug":"rat-neuronal-proteasome","display_name":"Rat cortical-neuron proteasome activity","entity_type_key":"protein_complex"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9a63c015-5a54-561f-8e95-356c3ddad196","slug":"rat-cortical-neuron-survival-without-trophic-factors","display_name":"Rat cortical-neuron survival after trophic-factor withdrawal","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pharmacological proteasome inhibition in rat neurons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a dietary fisetin-deficiency syndrome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Blocking downstream machinery prevented the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The flavonoid fisetin promotes nerve cell survival from trophic factor withdrawal by enhancement of proteasome activity. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18396148/ · DOI 10.1016/j.abb.2008.03.023","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2b681fd3-9464-5412-abed-c95b269df5c1","evidence_kind":"source_excerpt","locator":"Lines 360-366","start_line":360,"end_line":366,"excerpt":"## fisetin-proteasome-block\nBlocking downstream machinery prevented the response.\nProteasome inhibitors nearly abolished fisetin-supported survival of trophic-factor-deprived rat cortical neurons.\nModel: Pharmacological proteasome inhibition in rat neurons.\nLimitations: Not a dietary fisetin-deficiency syndrome.\nEvidence access: Primary abstract\nThe flavonoid fisetin promotes nerve cell survival from trophic factor withdrawal by enhancement of proteasome activity. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18396148/ · DOI 10.1016/j.abb.2008.03.023","model_system":"Pharmacological proteasome inhibition in rat neurons.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}