{"id":"471e9223-ca8f-5b9f-a336-a3a7d6935e63","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-bacterial-availability","predicate":"enables","statement":"In ergothioneine-controlled culture medium, wild-type Helicobacter pylori required available ergothioneine to outcompete its transporter-deficient strain.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b0f33230-0a12-5479-8145-7d4760898de6","mechanism_event_label":"External supply and uptake machinery act together.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"edcf6788-db74-5c2e-9e0e-2e3f3ba678ec","slug":"helicobacter-pylori-ergothioneine-competition","display_name":"Helicobacter pylori competitive fitness in ergothioneine-controlled medium","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b0f33230-0a12-5479-8145-7d4760898de6","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-bacterial-availability-event","event_type":"observed_relationship","label":"External supply and uptake machinery act together.","description":"In ergothioneine-controlled culture medium, wild-type Helicobacter pylori required available ergothioneine to outcompete its transporter-deficient strain.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"edcf6788-db74-5c2e-9e0e-2e3f3ba678ec","slug":"helicobacter-pylori-ergothioneine-competition","display_name":"Helicobacter pylori competitive fitness in ergothioneine-controlled medium","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"760990df-cc95-516c-ab87-e3ec02c81bf6","slug":"helicobacter-pylori-egtuv","display_name":"Helicobacter pylori EgtUV transporter","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Affinity-resin depletion and bacterial competition in vitro.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Controlled medium depletion; not a human nutritional diagnosis or infection outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"External supply and uptake machinery act together.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"An affinity-based depletion strategy for evaluating the effects of ergothioneine on bacterial physiology. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40068683/ · DOI 10.1016/j.chembiol.2025.02.004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"af90633c-1939-532c-810d-88f47941b12e","evidence_kind":"source_excerpt","locator":"Lines 248-254","start_line":248,"end_line":254,"excerpt":"## ergothioneine-bacterial-availability\nExternal supply and uptake machinery act together.\nIn ergothioneine-controlled culture medium, wild-type Helicobacter pylori required available ergothioneine to outcompete its transporter-deficient strain.\nModel: Affinity-resin depletion and bacterial competition in vitro.\nLimitations: Controlled medium depletion; not a human nutritional diagnosis or infection outcome.\nEvidence access: Primary abstract\nAn affinity-based depletion strategy for evaluating the effects of ergothioneine on bacterial physiology. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40068683/ · DOI 10.1016/j.chembiol.2025.02.004","model_system":"Affinity-resin depletion and bacterial competition in vitro.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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