{"id":"470b85d0-6605-55f9-a916-7f10b1b70316","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:dibasic-urinary-calcium-increase","predicate":"with_arginine_increased","statement":"In the same crossover study, lysine plus arginine significantly increased urinary calcium compared with the low-protein control diet.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0238134a-d70a-5ca8-a6ef-ac2feaebc24a","mechanism_event_label":"More urinary calcium accompanied the mixture; it cannot by itself be read as either bone loss or better bone health.","subject":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"object":{"id":"538fbe5c-11da-5aa5-990f-e8be1aa7bc5a","slug":"urinary-calcium-excretion","display_name":"Urinary calcium excretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0238134a-d70a-5ca8-a6ef-ac2feaebc24a","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:dibasic-urinary-calcium-increase-event","event_type":"observed_intervention","label":"More urinary calcium accompanied the mixture; it cannot by itself be read as either bone loss or better bone health.","description":"In the same crossover study, lysine plus arginine significantly increased urinary calcium compared with the low-protein control diet.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"538fbe5c-11da-5aa5-990f-e8be1aa7bc5a","slug":"urinary-calcium-excretion","display_name":"Urinary calcium excretion","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"cointervention","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"excreted_ion","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Randomized crossover feeding trial in 14 women","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No lysine-only arm or demonstrated long-term calcium-balance effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"More urinary calcium accompanied the mixture; it cannot by itself be read as either bone loss or better bone health.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[bihuniak2014] Supplementing a low-protein diet with dibasic amino acids increases urinary calcium excretion in young women (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC3927545/ DOI: 10.3945/jn.113.185009","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Kidney and urine","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"edc4060a-4407-5290-be06-8a7c9805f4a1","evidence_kind":"source_excerpt","locator":"Lines 779-787","start_line":779,"end_line":787,"excerpt":"### dibasic-urinary-calcium-increase\nIn the same crossover study, lysine plus arginine significantly increased urinary calcium compared with the low-protein control diet.\nPlain language: More urinary calcium accompanied the mixture; it cannot by itself be read as either bone loss or better bone health.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Kidney and urine\nexperimental_model: Randomized crossover feeding trial in 14 women\nlimitations: No lysine-only arm or demonstrated long-term calcium-balance effect.\n[bihuniak2014] Supplementing a low-protein diet with dibasic amino acids increases urinary calcium excretion in young women (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC3927545/ DOI: 10.3945/jn.113.185009","model_system":"Randomized crossover feeding trial in 14 women","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [bihuniak2014] Supplementing a low-protein diet with dibasic amino acids increases urinary calcium excretion in young women (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC3927545/ DOI: 10.3945/jn.113.185009","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[{"id":"f5abcf2c-b3de-55d0-89aa-dada70b27f41","title":"Urinary calcium changes differ across intervention contexts","kind":"context_difference","status":"open","why":"An acute calcium load with lysine differs from several days of lysine-plus-arginine on a low-protein diet. Urinary calcium alone does not settle net calcium balance or bone health.","resolution":"Unresolved; needs review.","created_at":"2026-09-17 07:46:03","record_type":"conflict","display_label":"Recorded conflict","record_url":"/conflicts/f5abcf2c-b3de-55d0-89aa-dada70b27f41","sides":[{"conflict_id":"f5abcf2c-b3de-55d0-89aa-dada70b27f41","ordinal":0,"label":"Dietary L-lysine and calcium metabolism in humans","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","start_line":759,"end_line":767,"quote":"### lysine-calcium-urinary-blunting\nIn healthy women, adding 400 mg lysine to a calcium load blunted the subsequent rise in urinary calcium; this pattern was not reported for the osteoporotic group.\nPlain language: Lysine altered short-term urinary calcium handling in one subgroup.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Kidney and urine\nexperimental_model: Acute calcium-load comparison; healthy and osteoporotic women\nlimitations: Acute load response does not establish long-term calcium balance or skeletal outcomes.\n[civitelli1992] Dietary L-lysine and calcium metabolism in humans (1992). https://pubmed.ncbi.nlm.nih.gov/1486246/","source_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","source_title":"L-Lysine: mechanism-first literature curation (2026-09-17)","claim_ids":["a9fe5b4f-8f78-5e95-bf6c-7e041cc14179"]},{"conflict_id":"f5abcf2c-b3de-55d0-89aa-dada70b27f41","ordinal":1,"label":"Supplementing a low-protein diet with dibasic amino acids increases urinary calcium excretion in young women","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","start_line":779,"end_line":787,"quote":"### dibasic-urinary-calcium-increase\nIn the same crossover study, lysine plus arginine significantly increased urinary calcium compared with the low-protein control diet.\nPlain language: More urinary calcium accompanied the mixture; it cannot by itself be read as either bone loss or better bone health.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Kidney and urine\nexperimental_model: Randomized crossover feeding trial in 14 women\nlimitations: No lysine-only arm or demonstrated long-term calcium-balance effect.\n[bihuniak2014] Supplementing a low-protein diet with dibasic amino acids increases urinary calcium excretion in young women (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC3927545/ DOI: 10.3945/jn.113.185009","source_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","source_title":"L-Lysine: mechanism-first literature curation (2026-09-17)","claim_ids":["470b85d0-6605-55f9-a916-7f10b1b70316"]}]}],"corrections":[],"research":null}