{"id":"46b3e222-f2db-520c-86c8-6cd273d0d284","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-human-bat-carrier","predicate":"associates_with","statement":"Human supraclavicular BAT SLC25A44 mRNA increased after cold exposure and correlated with UCP1 and BCKDHA expression.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a41a11f7-2cec-5a76-9508-fb63488b4055","mechanism_event_label":"Human tissue showed a coordinated change in the shared catabolic system.","subject":{"id":"feb1e3ed-472a-5b4b-9582-66fdbe359b78","slug":"human-cold-exposure","display_name":"Controlled cold exposure in the human BAT study","entity_type_key":"cellular_process"},"object":{"id":"7c282ac5-c4f9-513a-a7a0-15a07c7e5d4e","slug":"slc25a44","display_name":"Human mitochondrial BCAA carrier / SLC25A44","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a41a11f7-2cec-5a76-9508-fb63488b4055","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-human-bat-carrier-event","event_type":"observed_relationship","label":"Human tissue showed a coordinated change in the shared catabolic system.","description":"Human supraclavicular BAT SLC25A44 mRNA increased after cold exposure and correlated with UCP1 and BCKDHA expression.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"feb1e3ed-472a-5b4b-9582-66fdbe359b78","slug":"human-cold-exposure","display_name":"Controlled cold exposure in the human BAT study","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7c282ac5-c4f9-513a-a7a0-15a07c7e5d4e","slug":"slc25a44","display_name":"Human mitochondrial BCAA carrier / SLC25A44","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"29e3916c-76ec-50fe-a9b7-177cfec8a7c8","slug":"bckdha","display_name":"Branched-chain ketoacid dehydrogenase E1 alpha / BCKDHA","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cold-exposure BAT samples; expression correlations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Observational expression evidence, not transporter gene manipulation or proof of isoleucine-specific clearance in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Isoleucine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Human tissue showed a coordinated change in the shared catabolic system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"BCAA catabolism in brown fat controls energy homeostasis through SLC25A44. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31435015/ · DOI 10.1038/s41586-019-1503-x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"19f7b817-a0f4-5a03-9bd1-240ae86a9770","evidence_kind":"source_excerpt","locator":"Lines 58-64","start_line":58,"end_line":64,"excerpt":"## isoleucine-human-bat-carrier\nHuman tissue showed a coordinated change in the shared catabolic system.\nHuman supraclavicular BAT SLC25A44 mRNA increased after cold exposure and correlated with UCP1 and BCKDHA expression.\nModel: Human cold-exposure BAT samples; expression correlations.\nLimitations: Observational expression evidence, not transporter gene manipulation or proof of isoleucine-specific clearance in humans.\nEvidence access: Primary full text\nBCAA catabolism in brown fat controls energy homeostasis through SLC25A44. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31435015/ · DOI 10.1038/s41586-019-1503-x","model_system":"Human cold-exposure BAT samples; expression correlations.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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