{"id":"466bce25-cd04-5b82-be72-2fed86015211","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-asc-ubiquitination","predicate":"reduces_ubiquitination","statement":"Myricetin reduced ROS-dependent ASC ubiquitination and reduced ASC–NLRP3 interaction and ASC oligomerization.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fdc0cb1a-c4a9-5dc1-a149-a470bf3a308e","mechanism_event_label":"The two proteins did not show the same ubiquitination direction.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"a40b9e0e-0c37-5cad-bf62-a88f68468296","slug":"myricetin-asc-assay","display_name":"ASC/PYCARD in the myricetin ubiquitination assay; cell species unresolved in abstract","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fdc0cb1a-c4a9-5dc1-a149-a470bf3a308e","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-asc-ubiquitination-event","event_type":"observed_relationship","label":"The two proteins did not show the same ubiquitination direction.","description":"Myricetin reduced ROS-dependent ASC ubiquitination and reduced ASC–NLRP3 interaction and ASC oligomerization.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a40b9e0e-0c37-5cad-bf62-a88f68468296","slug":"myricetin-asc-assay","display_name":"ASC/PYCARD in the myricetin ubiquitination assay; cell species unresolved in abstract","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ac5ef071-80d2-521e-86fe-372eda174ffd","slug":"myricetin-nlrp3-assay","display_name":"NLRP3 in the myricetin ubiquitination assay; cell species unresolved in abstract","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cellular inflammasome assembly assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No specific E3 ligase or ubiquitin-chain linkage established by the accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The two proteins did not show the same ubiquitination direction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Myricetin inhibits NLRP3 inflammasome activation via reduction of ROS-dependent ubiquitination of ASC and promotion of ROS-independent NLRP3 ubiquitination. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30594691/ · DOI 10.1016/j.taap.2018.12.019","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1d1381fb-c821-5128-b82d-834099f3553b","evidence_kind":"source_excerpt","locator":"Lines 628-634","start_line":628,"end_line":634,"excerpt":"## myricetin-asc-ubiquitination\nThe two proteins did not show the same ubiquitination direction.\nMyricetin reduced ROS-dependent ASC ubiquitination and reduced ASC–NLRP3 interaction and ASC oligomerization.\nModel: Cellular inflammasome assembly assays.\nLimitations: No specific E3 ligase or ubiquitin-chain linkage established by the accessed abstract.\nEvidence access: Primary abstract\nMyricetin inhibits NLRP3 inflammasome activation via reduction of ROS-dependent ubiquitination of ASC and promotion of ROS-independent NLRP3 ubiquitination. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30594691/ · DOI 10.1016/j.taap.2018.12.019","model_system":"Cellular inflammasome assembly assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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