{"id":"465edd84-3da4-54ba-a68a-db4289c35347","stable_key":"4910e17b-aaec-52cd-b9e5-caca85d817ee:red-yeast-rice-ankaflavin-ampk","predicate":"increases_tested_phosphorylation_of","statement":"Adding ankaflavin increased hepatic AMPK phosphorylation in high-fat-diet-fed mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0a35f9e5-3218-554a-a2ed-ab8cab7c23da","mechanism_event_label":"A pigment changed an energy-regulation signal in mice.","subject":{"id":"92939faa-84d1-52e8-a70f-deefd2486d52","slug":"ankaflavin","display_name":"Ankaflavin","entity_type_key":"small_molecule"},"object":{"id":"3ae42ef3-e864-5c21-bdcf-03a3a5cc252e","slug":"mouse-hepatic-ampk-pigment-assay","display_name":"Mouse hepatic AMPK activity in the Monascus pigment study","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0a35f9e5-3218-554a-a2ed-ab8cab7c23da","stable_key":"4910e17b-aaec-52cd-b9e5-caca85d817ee:red-yeast-rice-ankaflavin-ampk-event","event_type":"observed_relationship","label":"A pigment changed an energy-regulation signal in mice.","description":"Adding ankaflavin increased hepatic AMPK phosphorylation in high-fat-diet-fed mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"92939faa-84d1-52e8-a70f-deefd2486d52","slug":"ankaflavin","display_name":"Ankaflavin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3ae42ef3-e864-5c21-bdcf-03a3a5cc252e","slug":"mouse-hepatic-ampk-pigment-assay","display_name":"Mouse hepatic AMPK activity in the Monascus pigment study","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"C57BL/6 mouse liver; exposure dose not available in the accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Phosphorylation is not proof of direct AMPK binding or a demonstrated effect of every red yeast rice product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Red yeast rice collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"red-yeast-rice","display_name":"Red yeast rice","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A pigment changed an energy-regulation signal in mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[24275089] Monascin and ankaflavin act as natural AMPK activators with PPARα agonist activity to down-regulate nonalcoholic steatohepatitis in high-fat diet-fed C57BL/6 mice. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24275089/ · DOI 10.1016/j.fct.2013.11.015","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ddc22750-0a12-562f-95eb-b539a7143aeb","evidence_kind":"source_excerpt","locator":"Lines 220-226","start_line":220,"end_line":226,"excerpt":"## red-yeast-rice-ankaflavin-ampk\nA pigment changed an energy-regulation signal in mice.\nAdding ankaflavin increased hepatic AMPK phosphorylation in high-fat-diet-fed mice.\nModel: C57BL/6 mouse liver; exposure dose not available in the accessed abstract.\nLimitations: Phosphorylation is not proof of direct AMPK binding or a demonstrated effect of every red yeast rice product.\nEvidence access: Primary abstract\n[24275089] Monascin and ankaflavin act as natural AMPK activators with PPARα agonist activity to down-regulate nonalcoholic steatohepatitis in high-fat diet-fed C57BL/6 mice. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24275089/ · DOI 10.1016/j.fct.2013.11.015","model_system":"C57BL/6 mouse liver; exposure dose not available in the accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"261a12bc-3d00-52a4-a5ec-70e174390596","stable_key":"import-4910e17b-aaec-52cd-b9e5-caca85d817ee","title":"Red yeast rice: constituents, mevalonate, CoQ and product-specific interactions (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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