{"id":"465d1d01-ab0f-5325-96d5-92bfeb90a914","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-defined-cell-tau","predicate":"reduces_assay_signal","statement":"The reagent reduced tau HTRF aggregation signals, while tau BiFC-positive cell frequency was unchanged.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c0dc4416-2652-5760-b339-91135f0ab01c","mechanism_event_label":"Two assays measured different aspects of the tau response.","subject":{"id":"d6140c5e-730e-5710-b502-424b7b4f6836","slug":"fungal-fulvic-acid-479-66-3","display_name":"Defined fungal fulvic acid / CAS 479-66-3","entity_type_key":"small_molecule"},"object":{"id":"f2de4af8-655d-5bb1-b98a-c01b7eba4ee3","slug":"human-hek293-tau-aggregation","display_name":"Tau aggregation readouts in human HEK293 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c0dc4416-2652-5760-b339-91135f0ab01c","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-defined-cell-tau-event","event_type":"observed_relationship","label":"Two assays measured different aspects of the tau response.","description":"The reagent reduced tau HTRF aggregation signals, while tau BiFC-positive cell frequency was unchanged.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d6140c5e-730e-5710-b502-424b7b4f6836","slug":"fungal-fulvic-acid-479-66-3","display_name":"Defined fungal fulvic acid / CAS 479-66-3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f2de4af8-655d-5bb1-b98a-c01b7eba4ee3","slug":"human-hek293-tau-aggregation","display_name":"Tau aggregation readouts in human HEK293 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e6e12feb-ecfe-5217-8cfd-5184a502cceb","slug":"mapt","display_name":"Human microtubule-associated protein tau / MAPT","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"2020 study; Cayman CAS 479-66-3 at 37 micromolar; HEK293 constructs or purified K18 tau as specified. Twelve-hour treatment following co-culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The methods identify a defined fungal compound, not an interchangeable environmental mixture. Supplier identity: https://www.caymanchem.com/product/19063/fulvic-acid . No clinical efficacy or CNS exposure was measured. Assay differences are retained rather than collapsed into universal suppression.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Two assays measured different aspects of the tau response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Effects of pharmacological modulators of α-synuclein and tau aggregation and internalization. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32732936/ · DOI 10.1038/s41598-020-69744-y","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"39cbef33-413a-575c-9d40-8f63dbb3685d","evidence_kind":"source_excerpt","locator":"Lines 172-178","start_line":172,"end_line":178,"excerpt":"## fulvic-acid-defined-cell-tau\nTwo assays measured different aspects of the tau response.\nThe reagent reduced tau HTRF aggregation signals, while tau BiFC-positive cell frequency was unchanged.\nModel: 2020 study; Cayman CAS 479-66-3 at 37 micromolar; HEK293 constructs or purified K18 tau as specified. Twelve-hour treatment following co-culture.\nLimitations: The methods identify a defined fungal compound, not an interchangeable environmental mixture. Supplier identity: https://www.caymanchem.com/product/19063/fulvic-acid . No clinical efficacy or CNS exposure was measured. Assay differences are retained rather than collapsed into universal suppression.\nEvidence access: Primary full text\nEffects of pharmacological modulators of α-synuclein and tau aggregation and internalization. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32732936/ · DOI 10.1038/s41598-020-69744-y","model_system":"2020 study; Cayman CAS 479-66-3 at 37 micromolar; HEK293 constructs or purified K18 tau as specified. Twelve-hour treatment following co-culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"52c2c3ee6bb23d943a40e1c39dd9de5cca7acd6efa72479cb824149e85cc7968","revision_id":"94dfc01b-c93e-55ff-8f48-bb2e9a1979f6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}