{"id":"46136cd7-e86e-52d8-b470-0f61569cb2d9","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-enz-bvitamin-cell-hydrolysis","predicate":"deficiency_reduces","statement":"Membrane lysates from cells cultured under zinc-deficient conditions had reduced extracellular B-vitamin phosphate-ester hydrolysis activity in the study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d327672c-f5f8-519c-9469-a4344c787c6a","mechanism_event_label":"Low zinc availability reduced the experimental preparations’ ability to remove phosphate groups from B vitamins.","subject":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"object":{"id":"0eede301-252b-5c75-b19d-54cb0e8a201d","slug":"b-vitamin-phosphate-hydrolysis","display_name":"Extracellular B-vitamin phosphate-ester hydrolysis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d327672c-f5f8-519c-9469-a4344c787c6a","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-enz-bvitamin-cell-hydrolysis-event","event_type":"biochemical_relationship","label":"Low zinc availability reduced the experimental preparations’ ability to remove phosphate groups from B vitamins.","description":"Membrane lysates from cells cultured under zinc-deficient conditions had reduced extracellular B-vitamin phosphate-ester hydrolysis activity in the study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"187db168-8028-5ce6-9f8b-4bc61ebad1a0","slug":"thiamine-diphosphate","display_name":"Thiamine diphosphate","entity_type_key":"small_molecule"},"role":"b1_phosphate_context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"b2_context","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"b6_phosphate_context","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"0eede301-252b-5c75-b19d-54cb0e8a201d","slug":"b-vitamin-phosphate-hydrolysis","display_name":"Extracellular B-vitamin phosphate-ester hydrolysis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Membrane lysates of transiently transfected or endogenously expressing cells; serum of rats fed zinc-deficient diets","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cells cultured under experimental zinc-deficient conditions; depletion method and concentrations not specified in the abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract-only extraction. Individual enzyme-reaction assignments, depletion concentrations/durations, quantitative effects and human clinical implications are not established here. Hydrolysis of extracellular vitamin phosphates is distinct from intracellular cofactor activation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Cell species not specified in abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Low zinc availability reduced the experimental preparations’ ability to remove phosphate groups from B vitamins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-enz-bvitamins2024] Rate of hydrolysis of the phosphate esters of B vitamins is reduced by zinc deficiency: In vitro and in vivo. (2024). https://pubmed.ncbi.nlm.nih.gov/39279493/ DOI: 10.1096/fj.202401207r","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured-cell membrane lysates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"b50cdae6-18ac-5904-9ad6-76cab9f741a3","evidence_kind":"source_excerpt","locator":"Lines 833-844","start_line":833,"end_line":844,"excerpt":"### zinc-enz-bvitamin-cell-hydrolysis\nMembrane lysates from cells cultured under zinc-deficient conditions had reduced extracellular B-vitamin phosphate-ester hydrolysis activity in the study.\nCondition category: nutrient_deficiency\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Low zinc availability reduced the experimental preparations’ ability to remove phosphate groups from B vitamins.\norganism: Cell species not specified in abstract\ntissue_or_cell_type: Cultured-cell membrane lysates\nexperimental_model: Membrane lysates of transiently transfected or endogenously expressing cells; serum of rats fed zinc-deficient diets\nlimitations: Abstract-only extraction. Individual enzyme-reaction assignments, depletion concentrations/durations, quantitative effects and human clinical implications are not established here. Hydrolysis of extracellular vitamin phosphates is distinct from intracellular cofactor activation.\nexposure: Cells cultured under experimental zinc-deficient conditions; depletion method and concentrations not specified in the abstract.\ncross_nutrient: true\n[zinc-enz-bvitamins2024] Rate of hydrolysis of the phosphate esters of B vitamins is reduced by zinc deficiency: In vitro and in vivo. (2024). https://pubmed.ncbi.nlm.nih.gov/39279493/ DOI: 10.1096/fj.202401207r","model_system":"Membrane lysates of transiently transfected or endogenously expressing cells; serum of rats fed zinc-deficient diets","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-enz-bvitamins2024] Rate of hydrolysis of the phosphate esters of B vitamins is reduced by zinc deficiency: In vitro and in vivo. (2024). https://pubmed.ncbi.nlm.nih.gov/39279493/ DOI: 10.1096/fj.202401207r","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2e731dd54477ec1254e97df3323e1208d38f1375effed19252e71ab4f600d13a","revision_id":"c72258b9-ac09-5408-9d44-a921ad1f96a3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}